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Titolo:
A panel of epitope-specific antibodies detects protein domains distributedthroughout human alpha-synuclein in Lewy bodies of Parkinson's disease
Autore:
Giasson, BI; Jakes, R; Goedert, M; Duda, JE; Leight, S; Trojanowski, JQ; Lee, VMY;
Indirizzi:
Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 & Lab Med, Philadelphia, PA 19104 USA MRC, Mol Biol Lab, Cambridge CB2 2QH, England MRC Cambridge England CB2 2QH , Mol Biol Lab, Cambridge CB2 2QH, England
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 4, volume: 59, anno: 2000,
pagine: 528 - 533
SICI:
0360-4012(20000215)59:4<528:APOEAD>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLE SYSTEM ATROPHY; A-BETA COMPONENT; GLIAL CYTOPLASMIC INCLUSIONS; CENTRAL-NERVOUS-SYSTEM; ALZHEIMERS-DISEASE; NACP/ALPHA-SYNUCLEIN; BODY DISEASE; PRESYNAPTIC PROTEIN; PRECURSOR PROTEIN; 14-KDA PROTEIN;
Keywords:
antibodies; Lewy body; Parkinson's disease; alpha-synuclein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, VMY Hosp Univ Penn, Dept Pathol & Lab Med, Dept Neurodegenerat Dis Res, Philadelphia, PA 19104 USA Hosp Univ Penn Philadelphia PA USA 19104 ladelphia, PA 19104 USA
Citazione:
B.I. Giasson et al., "A panel of epitope-specific antibodies detects protein domains distributedthroughout human alpha-synuclein in Lewy bodies of Parkinson's disease", J NEUROSC R, 59(4), 2000, pp. 528-533

Abstract

To facilitate studies of the normal biology of alpha-synuclein, a member of a family of neuronal proteins of unknown function, and to elucidate the role of alpha-synuclein pathologies in neurodegenerative diseases, we generated and characterized a panel of anti-synuclein antibodies. Mere we demonstrate that these antibodies recognize defined epitopes spanning the entire length of human alpha-synuclein, and that some of these antibodies also cross-react with zebra finch and rodent synucleins. Since alpha-synuclein has been reported to be a major component of Lewy bodies (LBs) in Parkinson's disease (PD), dementia with LBs and common variants of Alzheimer's disease, we performed immunohistochemical studies showing that these antibodies labelnumerous LBs in the PD substantia nigra, thereby localizing protein domains throughout human alpha-synuclein in LBs. Taken together, our data indicate that this panel of antibodies can be exploited to probe the normal biology of alpha-synuclein as well as the role of pathological forms of this protein in PD and related neurodegenerative synucleinopathies. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:41:14