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Titolo:
Persistence of Heterorhabditis infective juveniles in soil: Comparison of extraction and infectivity measurements
Autore:
Hass, B; Griffin, CT; Downes, MJ;
Indirizzi:
Natl Univ Ireland, Dept Biol, Maynooth, Kildare, Ireland Natl Univ Ireland Maynooth Kildare Ireland l, Maynooth, Kildare, Ireland
Titolo Testata:
JOURNAL OF NEMATOLOGY
fascicolo: 4, volume: 31, anno: 1999,
pagine: 508 - 516
SICI:
0022-300X(199912)31:4<508:POHIJI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTOMOPATHOGENIC NEMATODE PERSISTENCE; STEINERNEMA-FELTIAE; POPULATIONS; TEMPERATURE; STORAGE; NUMBER; GEN;
Keywords:
Baermann funnel; centrifugal flotation; dissection; entomopathogenic nematode; Heterorhabditis bacteriophora; Heterorhabditis megidis; infectivity; LD50; nematode; persistence; soil baiting; soil dilution; soil ecology; soil extraction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Hass, B Univ Kiel, Dept Biotechnol & Biol Control, Inst Phytopathol, D-24223 Raisdorf, Germany Univ Kiel Raisdorf Germany D-24223 ol, D-24223 Raisdorf, Germany
Citazione:
B. Hass et al., "Persistence of Heterorhabditis infective juveniles in soil: Comparison of extraction and infectivity measurements", J NEMATOL, 31(4), 1999, pp. 508-516

Abstract

The persistence of Heterorhabditis megidis in soil was studied over a 4-week period. On days 0, 2, 14, and 28, infective juveniles (IJ) were extracted by centrifugal flotation, Baermann funnel, and baiting of soil with Tenebrio molitor larvae, which were then dissected. Extraction efficiencies on day 0 were 82% by centrifugal flotation, 56% by Baermann funnel, and 19.8% by bait insect. The relative efficiency of the three methods changed over time. The relationship between the-density of nematodes in the soil and the proportion recovered by dissection was non-linear. Up to a dose of approximately 60 IJ/insect, less than 12% became established, while at higher doses (up to 200 IJ/insect) the invasion efficiency was 23%. Mortality of bait insects increased from day 0 to day 2, but decreased to day 28. A novel method of assessing soil pathogenicity by preparing a soil density series and calculating the dose of soil or IJ that kills 50% of the bait insects gave a similar pattern. This method is recommended as a means of tracking changes in pathogenicity over time when bait insect mortality in undiluted soil is at or near 100%. Two methods of preparing a series of Heterorhabditis IJ densities in soil, either by diluting the soil itself with IJ-free soil or byadding diluted suspensions of IJ to the soil, resulted in the same bait insect mortalities.

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Documento generato il 27/09/20 alle ore 13:39:50