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Titolo:
CcpC, a novel regulator of the LysR family required for glucose repressionof the citB gene in Bacillus subtilis
Autore:
Jourlin-Castelli, C; Mani, N; Nakano, MM; Sonenshein, AL;
Indirizzi:
Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02111 USA TuftsUniv Boston MA USA 02111 Mol Biol & Microbiol, Boston, MA 02111 USA Oregon Grad Inst Sci & Technol, Dept Biochem & Mol Biol, Portland, OR 97291 USA Oregon Grad Inst Sci & Technol Portland OR USA 97291 rtland, OR 97291 USA
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 4, volume: 295, anno: 2000,
pagine: 865 - 878
SICI:
0022-2836(20000128)295:4<865:CANROT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATABOLITE REPRESSION; TRANSCRIPTIONAL REGULATION; ESCHERICHIA-COLI; OPERON; EXPRESSION; INITIATION; ACONITASE; SEQUENCE; PROMOTER; ENCODES;
Keywords:
Bacillus subtilis; aconitase; catabolite repression; LysR family;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Sonenshein, AL Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, 136 Harrison Ave, Boston, MA 02111 USA Tufts Univ 136 Harrison Ave Boston MA USA 02111A 02111 USA
Citazione:
C. Jourlin-Castelli et al., "CcpC, a novel regulator of the LysR family required for glucose repressionof the citB gene in Bacillus subtilis", J MOL BIOL, 295(4), 2000, pp. 865-878

Abstract

Synergistic carbon catabolite repression of the Bacillus subtilis aconitase (citB) gene by glucose and a source of 2-ketoglutarate is dependent on DNA sequences located upstream of the gene. Mutations in a dyad symmetry element centered at position -66 and in a repeat of the downstream arm of the dyad symmetry at position -27 cause derepressed citB expression. In this work, a protein able to bind to a DNA fragment containing these elements was purified and identified. This protein, named CcpC (Catabolite control protein C), shares sequence similarity with members of the LysR family of transcriptional regulators. In addition to binding to the citB promoter, CcpC bound to the promoter of the citZ gene, which encodes the cell's major citrate synthase and is subject to carbon catabolite repression. In a ccpC null mutant, expression of both citB and citZ was derepressed in glucose-glutamine minimal medium, indicating that CcpC is a negative regulator of citB and citZ gene expression. DNase I footprinting experiments showed that CcpC bindsto two sites within the citB promoter region, corresponding to the dyad symmetry and -27 elements. In the presence of citrate, a putative inducer, only the dyad symmetry element was fully protected by CcpC. When the dyad symmetry element was mutated, CcpC was no longer able to bind to either the dyad symmetry or -27 elements. Repression of citB and citZ gene expression during anaerobiosis also proved to be mediated by CcpC. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:13:51