Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Phenotypical characterization of the neurons expressing the D1 and D2 dopamine receptors in the monkey striatum
Autore:
Aubert, I; Ghorayeb, I; Normand, E; Bloch, B;
Indirizzi:
Univ Bordeaux 2, CNRS, UMR 5541, Lab Histol Embryol, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 mbryol, F-33076 Bordeaux, France
Titolo Testata:
JOURNAL OF COMPARATIVE NEUROLOGY
fascicolo: 1, volume: 418, anno: 2000,
pagine: 22 - 32
SICI:
0021-9967(20000228)418:1<22:PCOTNE>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; PREPROENKEPHALIN MESSENGER-RNA; CENTRAL NERVOUS-SYSTEM; GENE-EXPRESSION; BASAL GANGLIA; RAT-BRAIN; INSITU HYBRIDIZATION; PARKINSONS-DISEASE; STRIATONIGRAL NEURONS; D2-DOPAMINE RECEPTOR;
Keywords:
monkey basal ganglia; in situ hybridization; preproenkephalin A; substance P; dopamine receptors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Aubert, I Univ Bordeaux 2, CNRS, UMR 5541, Lab Histol Embryol, 146 Rue LeoSaignat, F-33076 Bordeaux, France Univ Bordeaux 2 146 Rue Leo Saignat Bordeaux France F-33076 nce
Citazione:
I. Aubert et al., "Phenotypical characterization of the neurons expressing the D1 and D2 dopamine receptors in the monkey striatum", J COMP NEUR, 418(1), 2000, pp. 22-32

Abstract

The striatum is regulated by dopaminergic inputs from the substantia nigra. Several anatomical studies using in situ hybridization have demonstrated that; in rodents, dopamine D1 and D2 receptors are segregated into distinctstriatal efferent populations: dopamine D1 receptor into gamma-aminobutyric acid (GABA)/substance P striatonigral neurons, and dopamine D2 receptor into GABA/enkephalin striatopallidal neurons. The existence of such a segregation has not been investigated in primates. Therefore, to quantify the efferent striatal GABAergic neurons in the adult Cynomolgus monkey, we detected GAD(67) mRNA expression while considering that only a minority of the GABAergic population is composed of interneurons. To characterize the peptidergic phenotype of the neurons expressing dopamine D1 or D2 receptors, we examined the mRNA coding for these receptors in the striatum at the cellular level using single- and double in situ hybridization with digoxigenin and S-35 ribonucleotide probes. Double in situ hybridization demonstrated a high coexpression of dopamine D1 receptor and substance P mRNAs (91-99%) as wellas dopamine D2 receptor and preproenkephalin A mRNAs (96-99%) in medium-sized neurons throughout the nucleus caudatus, putamen, and nucleus accumbens. Only a small subpopulation (2-5%) of the neurons that contained dopamine D1 receptor mRNA also expressed dopamine D2 receptor mRNA in all regions. Large-sized neurons known to be cholinergic expressed D2R mRNA. However, within the nucleus basalis of Meynert, the large cholinergic neurons expressedD2R mRNA, but the neurons producing enkephalin expressed neither D1R nor D2R mRNA. These results demonstrate that the striatal organizational patternof D1 and D2 receptor segregation in distinct neuronal populations described in rodent also exists in primate. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 18:31:10