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Titolo:
Genomic variations in echovirus 30 persistent isolates recovered from a chronically infected immunodeficient child and comparison with the reference strain
Autore:
Bailly, JL; Chambon, M; Henquell, C; Icart, J; Peigue-Lafeuille, H;
Indirizzi:
UFR Med, Virol Lab, F-63002 Clermont Ferrand 1, France UFR Med Clermont Ferrand France 1 ab, F-63002 Clermont Ferrand 1, France CHU Rangueil, Serv Bacteriol Virol Hyg, F-31403 Toulouse, France CHU Rangueil Toulouse France F-31403 Virol Hyg, F-31403 Toulouse, France
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 2, volume: 38, anno: 2000,
pagine: 552 - 557
SICI:
0095-1137(200002)38:2<552:GVIE3P>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARTILAGE-HAIR HYPOPLASIA; ECHO VIRUS TYPE-25; ASEPTIC-MENINGITIS; POLIOVIRUS; SUBSTITUTIONS; POLIOMYELITIS; REPLICATION; ENTEROVIRUS; RESOLUTION; SEQUENCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Bailly, JL UFR Med, Virol Lab, 28 Pl Henri Dunant,BP38, F-63002 Clermont Ferrand 1, France UFR Med 28 Pl Henri Dunant,BP38 Clermont Ferrand France 1ance
Citazione:
J.L. Bailly et al., "Genomic variations in echovirus 30 persistent isolates recovered from a chronically infected immunodeficient child and comparison with the reference strain", J CLIN MICR, 38(2), 2000, pp. 552-557

Abstract

Seven sequential isolates of echovirus type 30 (EV30) were recovered over 22 months from a child with severe combined immune deficiency syndrome. Thenucleotide sequences of the 5' halves of the genomes (4,400 nucleotides) of the first (S1) and last (S7) isolates were determined and compared with that of the EV30 Bastianni reference strain, also determined in this study. In genome regions P1 and P2, 101 variations were identified between the twoisolates. Synonymous differences far outnumbered nonsynonymous differences. Amino acid changes affected both capsid and nonstructural polypeptides (particularly 2B), The VP1 nucleotide sequences of the seven isolates were determined to analyze genome evolution during the chronic infection. In the phylogenetic tree, the seven isolates were directly related to the prototypestrain in an individual monophyletic group, strongly suggesting that the chronic infection in the child arose from a single persistent EV30 isolate. Four lineages were observed in the persistent isolates. Isolates S2, S4, S5, and S6 were close relatives of one another, whereas isolates S1 and S3 formed individual lineages. Isolate S7, distantly related to all other isolates! formed the fourth lineage. These findings suggest the quasispecies nature of the genomes of the seven sequential EV30 isolates. Grouping of persistent isolates on the basis of replicative capacities a as consistent with phylogenetic relationships. Overall, the results indicate that genetically related EV30 variants with different replicative capacities coexisted in a carrier state, probably in the gastrointestinal tract, during the infection of the child.

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Documento generato il 20/10/20 alle ore 05:28:43