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Titolo:
Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures
Autore:
Kempf, VAJ; Trebesius, K; Autenrieth, IB;
Indirizzi:
Univ Munich, Max Von Pettenkofer Inst Hyg & Med Microbiol, D-80336 Munich,Germany Univ Munich Munich Germany D-80336 Med Microbiol, D-80336 Munich,Germany
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 2, volume: 38, anno: 2000,
pagine: 830 - 838
SICI:
0095-1137(200002)38:2<830:FISHAR>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; STREPTOCOCCUS-PNEUMONIAE; BACTERIAL TRANSLOCATION; STAPHYLOCOCCUS-AUREUS; MICROBIAL DNA; PCR; BACTEREMIA; PROBES; INFECTIONS; PREVALENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Autenrieth, IB Univ Munich, Max Von Pettenkofer Inst Hyg & Med Microbiol, Pettenkoferstr 9A, D-80336 Munich, Germany Univ Munich Pettenkoferstr 9A Munich Germany D-80336 rmany
Citazione:
V.A.J. Kempf et al., "Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures", J CLIN MICR, 38(2), 2000, pp. 830-838

Abstract

Using fluorescent in situ hybridization (FISH) with rRNA-targeted fluorescently labelled oligonucleotide probes, pathogens were rapidly detected and identified in positive blood culture bottles without cultivation and biotyping. In this study, 115 blood cultures with a positive growth index as determined by a continuous-reading automated blood culture system were examinedby both conventional laboratory methods and FISH. For this purpose, oligonucleotide probes that allowed identification of approximately 95% of those pathogens typically associated dth bacteremia were produced. The sensitivity and specificity of these probes were 100%. From all 115 blood cultures, microorganisms were grown after 1 day and identification to the family, genus, or species level was achieved after 1 to 3 days while 111 samples (96.5%) were similarly identified by FISH within 2.5 h. Staphylococci were identified in 62 of 62 samples, streptococci and enterococci were identified in 19 of 20 samples, gram-negative rods were identified in 28 of 30 samples, and fungi were identified in two of two samples. Thus, FISH is an appropriatemethod for identification of pathogens grown in blood cultures from septicemic patients.

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Documento generato il 20/09/20 alle ore 06:24:59