Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A genomic approach of the hepatitis C virus generates a protein interaction map
Autore:
Flajolet, M; Rotondo, G; Daviet, L; Bergametti, F; Inchauspe, G; Tiollais, P; Transy, C; Legrain, P;
Indirizzi:
Inst Pasteur, Unite Genet Interact Macromol, CNRS, URA 1300, F-75724 Paris15, France Inst Pasteur Paris France 15 ol, CNRS, URA 1300, F-75724 Paris15, France Inst Pasteur, INSERM, U163, F-75724 Paris, France Inst Pasteur Paris France F-75724 r, INSERM, U163, F-75724 Paris, France INSERM, U271, F-69003 Lyon, France INSERM Lyon France F-69003INSERM, U271, F-69003 Lyon, France
Titolo Testata:
GENE
fascicolo: 1-2, volume: 242, anno: 2000,
pagine: 369 - 379
SICI:
0378-1119(20000125)242:1-2<369:AGAOTH>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT RNA-POLYMERASE; SERINE-TYPE PROTEINASE; COMPLEX-FORMATION; CORE PROTEIN; NONSTRUCTURAL PROTEIN-3; NS3 PROTEASE; NS4A PROTEIN; IN-VITRO; YEAST; IDENTIFICATION;
Keywords:
polyprotein; protein domains; random libraries; two-hybrid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Legrain, P Inst Pasteur, Unite Genet Interact Macromol, CNRS, URA 1300, 25Rue Dr Roux, F-75724 Paris 15, France Inst Pasteur 25 Rue Dr Roux Paris France 15 4 Paris 15, France
Citazione:
M. Flajolet et al., "A genomic approach of the hepatitis C virus generates a protein interaction map", GENE, 242(1-2), 2000, pp. 369-379

Abstract

The hepatitis C virus (HCV) causes severe liver disease, including liver cancer. A vaccine preventing HCV infection has not yet been developed, and, given the increasing number of infected people, this virus is now considered a major public-health problem. The HCV genome is a plus-stranded RNA thatencodes a single polyprotein processed into at least 10 mature polypeptides. So far, only the interaction between the protease NS3 and its cofactor, NS4A, which is involved in the processing of the non-structural region, hasbeen extensively studied. Our work was aimed at constructing a protein interaction map of HCV. A classical two-hybrid system failed to detect any interactions between mature HCV polypeptides, suggesting incorrect folding, expression or targetting of these proteins. We therefore developed a two-hybrid strategy, based on exhaustive screens of a random genomic HCV library. Using this method, we found known interactions, such as the capsid homodimerand the protease dimer, NS3-NS4A, as well as several novel interactions such as NS4A-NS2. Thus, our results are consistent with the idea that the useof a random genomic HCV library allows the selection of correctly folded viral protein fragments. Interacting domains of the viral polyprotein an identified, opening the possibility of developing specific anti-viral agents, based on their ability to modulate these interactions. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:57:13