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Titolo:
TAFs revisited: more data reveal new twists and confirm old ideas
Autore:
Albright, SR; Tjian, R;
Indirizzi:
Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 ll Biol, Berkeley, CA 94720 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 242, anno: 2000,
pagine: 1 - 13
SICI:
0378-1119(20000125)242:1-2<1:TRMDRN>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; TATA-BINDING-PROTEIN; MEDIATE TRANSCRIPTIONAL ACTIVATION; RESOLUTION REFINED STRUCTURE; YEAST TFIIA/TBP/DNA COMPLEX; HUMAN TFIID COMPLEX; HISTONE-LIKE TAFS; CELL-FREE SYSTEM; CRYSTAL-STRUCTURE; MOLECULAR-CLONING;
Keywords:
coactivator; gene profiling; RNA polymerase II; TBP-associated factors; TFIID; transcription;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
133
Recensione:
Indirizzi per estratti:
Indirizzo: Tjian, R Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA Univ Calif Berkeley Berkeley CA USA 94720 Berkeley, CA 94720 USA
Citazione:
S.R. Albright e R. Tjian, "TAFs revisited: more data reveal new twists and confirm old ideas", GENE, 242(1-2), 2000, pp. 1-13

Abstract

Synthesis of messenger RNA by RNA polymerase II requires the combined activities of more than 70 polypeptides. Coordinating the interaction of these proteins is the basal transcription factor TFIID, which recognizes the corepromoter and supplies a scaffolding upon which the rest of the transcriptional machinery can assemble. A multisubunit complex, TFIID consists of the TATA-binding protein (TBP) and several TBP-associated factors (TAFs), whoseprimary sequences are well-conserved from yeast to humans. Data from reconstituted cell-free transcription systems and binary interaction assays suggest that the TAF subunits can function as promoter-recognition factors, as coactivators capable of transducing signals from enhancer-bound activators to the basal machinery, and even as enzymatic modifiers of other proteins. Whether TAFs function similarly in vivo, however, has been an open question. Initial characterization of yeast bearing mutations in particular TAFs seemingly indicated that, unlike the situation in vitro, TAFs played only a minor role in transcriptional regulation in vivo. However, reconsideration of this data in light of more recent results from yeast and other organisms reveals considerable convergence between the models derived from in vitro experiments and those derived from in vivo studies. In particular, there is an emerging consensus that TAFs represent one of several classes of coactivators that participate in transcriptional activation in vivo. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

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Documento generato il 22/09/20 alle ore 13:40:49