Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
sigma Receptor ligands attenuate N-methyl-D-aspartate cytotoxicity in dopaminergic neurons of mesencephalic slice cultures
Autore:
Shimazu, S; Katsuki, H; Takenaka, C; Tomita, M; Kume, T; Kaneko, S; Akaike, A;
Indirizzi:
Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Pharmacol, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Pharmacol, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Neuropharmacol, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 pharmacol, Sakyo Ku, Kyoto 6068501, Japan
Titolo Testata:
EUROPEAN JOURNAL OF PHARMACOLOGY
fascicolo: 2, volume: 388, anno: 2000,
pagine: 139 - 146
SICI:
0014-2999(20000128)388:2<139:SRLANC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG BRAIN; BINDING-SITES; MEDIATED NEUROPROTECTION; GLUTAMATE NEUROTOXICITY; CORTICAL-NEURONS; RAT-BRAIN; NMDA; INHIBITION; EXPRESSION; MECHANISMS;
Keywords:
sigma receptor; organotypic slice culture; mesencephalon; N-methyl-D-aspartate (NMDA); dopaminergic neuron;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Akaike, A Kyoto Univ, Grad Sch Pharmaceut Sci, Dept Pharmacol, Sakyo Ku, 46-29 Yoshida Shimoadachi Cho, Kyoto 6068501, Japan Kyoto Univ 46-29 YoshidaShimoadachi Cho Kyoto Japan 6068501 an
Citazione:
S. Shimazu et al., "sigma Receptor ligands attenuate N-methyl-D-aspartate cytotoxicity in dopaminergic neurons of mesencephalic slice cultures", EUR J PHARM, 388(2), 2000, pp. 139-146

Abstract

We investigated the potential neuroprotective effects of several a receptor ligands in organotypic midbrain slice cultures as an excitotoxicity modelsystem. When challenged with 100-mu M N-methyl-D-aspartate (NMDA) for 24 h, dopaminergic neurons in midbrain slice cultures degenerated, and this wasprevented by (5R, 10S)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,b]-cyclohepten-5,10-imine (MK-801; 1-10 mu M). Concomitant application of ifenprodil (1-10 mu M) or haloperidol (1-10 mu M), both of which are high-affinity sigma receptor ligands, significantly attenuated the neurotoxicity of 100 mu M NMDA. The sigma(1) receptor-selective ligand (+)-N-allylnormetazocine ((+)-SKF 10047; 1-10 mu M) was also effective in attenuating the toxicity of NMDA. The effect of R(-)-N-(3-phenyl-1-propyl)-1-phenyl-2-aminopropane hydrochloride ((-)-PPAP), a sigma receptor ligand with negligible affinity for thephencyclidine site of NMDA receptors, was also examined. (-)-PPAP (3-100 mu M) caused a concentration-dependent reduction of NMDA cytotoxicity, with significant protection at concentrations of 30 and 100 mu M. In contrast, ()-SKF 10047 (10 mu M) and (-)-PPAP (100 mu M) showed no protective effectsagainst cell death induced by the Ca2+ ionophore ionomycin (1-3 mu M). These results indicate that sigma receptor ligands attenuate the cytotoxic effects of NMDA on midbrain dopaminergic neurons, possibly via inhibition of NMDA receptor functions. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 09:11:02