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Titolo:
Forced expression of a dominant-negative chimeric tropomyosin causes abnormal motile behavior during cell division
Autore:
Wong, K; Wessels, D; Krob, SL; Matveia, AR; Lin, JLC; Soll, DR; Lin, JJC;
Indirizzi:
Univ Iowa, Dept Sci Biol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 , Dept Sci Biol, Iowa City, IA 52242 USA
Titolo Testata:
CELL MOTILITY AND THE CYTOSKELETON
fascicolo: 2, volume: 45, anno: 2000,
pagine: 121 - 132
SICI:
0886-1544(200002)45:2<121:FEOADC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; TRANSFORMED-CELLS; SACCHAROMYCES-CEREVISIAE; SPINDLE ORIENTATION; NONMUSCLE CELLS; BUDDING YEAST; RAS ONCOGENE; GENE TPM3; ISOFORMS; ACTIN;
Keywords:
cytokinesis; blebbing; misalignment of mitotic spindles; computer-assisted 2D and 3D dynamic image analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Lin, JJC Univ Iowa, Dept Sci Biol, 138 Biol Bldg, Iowa City, IA 52242 USA Univ Iowa 138 Biol Bldg Iowa City IA USA 52242 City, IA 52242 USA
Citazione:
K. Wong et al., "Forced expression of a dominant-negative chimeric tropomyosin causes abnormal motile behavior during cell division", CELL MOTIL, 45(2), 2000, pp. 121-132

Abstract

To further investigate the phenotypic consequences of misexpression, we have compared mitotic spindle organization and dynamic 2D and 3D shape changes during mitosis in normal cells and in a hTM5/3 misexpressing (mutant) cell line. Immunofluorescence microscopy of wild type and mutant cells stainedwith monoclonal anti-tubulin antibody revealed that the overall structuresof mitotic spindles were not significantly different. However, the axis ofthe mitotic spindle in mutant cells was more frequently misaligned with the long axis of the cell than that of wild type cells. To assess behavioral differences during mitosis, wild type and mutant cells were reconstructed in 2D and 3D and motion analyzed with the computer-assisted 2D and 3D Dynamic Image Analysis Systems (2D-DIAS, 3D-DIAS). Mutant cells abnormally formedlarge numbers of blebs during the later stages of mitosis and took longer to proceed from the start of anaphase to the start of cytokinesis. Furthermore, each mutant cell undergoing mitosis exhibited greater shape complexitythan wild type cells, and in every case lifted one of the two evolving daughter cells off the substratum and abnormally twisted. These results demonstrate that misexpression of hTM5/3 in CHO cells leads to morphological instability during mitosis. Misexpression of hTM5/3 interferes with normal tropomyosin function, suggesting in turn that tropomyosin plays a role through its interaction with actin microfilaments in the regulation of the contractile ring, in the localized suppression of blebbing, in the maintenance of polarity and spatial symmetry during cytokinesis, and in cell spreading after cytokinesis is complete. Cell Motil. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 15:51:49