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Titolo:
Hyperoxia synergistically increases TNF-alpha-induced interleukin-8 gene expression in A549 cells
Autore:
Allen, GL; Menendez, IY; Ryan, MA; Mazor, RL; Wispe, JR; Fiedler, MA; Wong, HR;
Indirizzi:
Childrens Hosp, Med Ctr, Div Crit Care Med OSB5, Res Fdn, Cincinnati, OH 45229 USA Childrens Hosp Cincinnati OH USA 45229 Res Fdn, Cincinnati, OH 45229 USA Childrens Hosp, Div Pulm Biol, Res Fdn, Cincinnati, OH 45229 USA ChildrensHosp Cincinnati OH USA 45229 Res Fdn, Cincinnati, OH 45229 USA Childrens Hosp, Div Pulm Med, Res Fdn, Cincinnati, OH 45229 USA Childrens Hosp Cincinnati OH USA 45229 Res Fdn, Cincinnati, OH 45229 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 2, volume: 278, anno: 2000,
pagine: L253 - L260
SICI:
1040-0605(200002)278:2<L253:HSITIG>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY-DISTRESS-SYNDROME; NF-KAPPA-B; OXYGEN-TOXICITY; INFLAMMATORY CYTOKINES; ALVEOLAR MACROPHAGES; CHEMOTACTIC FACTORS; LUNG; ACTIVATION; INJURY; BLOOD;
Keywords:
respiratory epithelium; inflammation; chemokines; tumor necrosis factor-alpha; nuclear factor-kappa B; nuclear factor-interleukin-6;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Wong, HR Childrens Hosp, Med Ctr, Div Crit Care Med OSB5, Res Fdn, 3333 Burnet Ave,Cincinnati, OH 45229 USA Childrens Hosp 3333 Burnet Ave CincinnatiOH USA 45229 45229 USA
Citazione:
G.L. Allen et al., "Hyperoxia synergistically increases TNF-alpha-induced interleukin-8 gene expression in A549 cells", AM J P-LUNG, 278(2), 2000, pp. L253-L260

Abstract

Interleukin (IL)-8 is an important mediator of acute lung injury. Hyperoxia induces IL-8 production in some cell types, but its effect on IL-8 gene expression in respiratory epithelium is not well described. In addition, IL-8 gene expression resulting from the combined effects of hyperoxia and proinflammatory cytokines has not been well characterized. We treated cultured respiratory epithelial-like cells (A549 cells) with hyperoxia alone, tumor necrosis factor (TNF)-alpha alone, or the combination of TNF-alpha and hyperoxia and evaluated IL-8 gene expression. Hyperoxia alone had a minimal effect on IL-8 gene expression, and TNF-alpha alone increased IL-8 gene expression in a time-dependent manner. In contrast, the combination of TNF-alpha and hyperoxia synergistically increased IL-8 gene expression as measured byELISA (TNF-alpha alone for 24 h = 769 +/- 89 pg/ml vs, hyperoxia + TNF-alpha for 24 h = 1,189 +/- 89 pg/ml) and Northern blot analyses. Experiments involving IL-8 promoter-reporter assays, electromobility shift assays, and Western blot analyses demonstrated that hyperoxia augmented TNF-alpha-mediated activation of the IL-8 promoter by a nuclear factor (NF)-kappa B-dependent mechanism and increased the duration of NF-kappa B nuclear translocationafter concomitant treatment with TNF-alpha. Additional reporter gene assays demonstrated, however, that increased activation of NF-kappa B does not fully account for the synergistic effect of hyperoxia and that the NF-IL-6 site in the IL-8 promoter is also required for the synergistic effect of hyperoxia. We conclude that hyperoxia alone has a minimal effect on IL-8 gene expression but synergistically increases IL-8 gene expression in the presence of TNF-alpha by a mechanism involving cooperative interaction between the transcription factors NF-kappa B and NF-IL-6.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 02:57:58