Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RPTP mu and protein tyrosine phosphorylation regulate K+ channel mRNA expression in adult cardiac myocytes
Autore:
Hershman, KM; Levitan, ES;
Indirizzi:
Univ Pittsburgh, Dept Pharmacol, Pittsburgh, PA 15261 USA Univ PittsburghPittsburgh PA USA 15261 armacol, Pittsburgh, PA 15261 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 2, volume: 278, anno: 2000,
pagine: C397 - C403
SICI:
0363-6143(200002)278:2<C397:RMAPTP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLONAL PITUITARY-CELLS; PHOSPHATASE PTP-MU; GENE-EXPRESSION; POTASSIUM CHANNEL; MESSENGER-RNA; IN-VIVO; KAPPA-B; TRANSCRIPTION; DEXAMETHASONE; CADHERINS;
Keywords:
receptor-type protein tyrosine phosphatase mu; cell-cell contact; rat ventricular myocyte; voltage-gated potassium channel; gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Levitan, ES Univ Pittsburgh, Dept Pharmacol, E1351 Biomed Sci Tower, Pittsburgh, PA 15261 USA Univ Pittsburgh E1351 Biomed Sci Tower Pittsburgh PA USA 15261
Citazione:
K.M. Hershman e E.S. Levitan, "RPTP mu and protein tyrosine phosphorylation regulate K+ channel mRNA expression in adult cardiac myocytes", AM J P-CELL, 278(2), 2000, pp. C397-C403

Abstract

Previously, we reported that cell-cell contact regulates K+ channel mRNA expression in cultured adult rat cardiac myocytes. Here we show that exposing cardiac myocytes to tyrosine kinase inhibitors (genistein, tyrphostin A25), but not inactive analogs, prevents downregulation of Kv1.5 mRNA and upregulation of Kv4.2 mRNA normally observed when they are cultured under low-density conditions. Furthermore, cardiac myocytes cocultured with cells thatendogenously (Mv 1 Lu) or heterologously (Chinese hamster ovary cells) express the receptor-type protein tyrosine phosphatase mu (RPTP mu) display Kv1.5 mRNA levels paralleling that which was observed in myocytes cultured under high-density conditions and in intact tissue. In contrast, myocytes cocultured with control cells failed to produce this response. Finally, it is shown that Kv4.2 mRNA expression is unaffected by RPTP mu. These findings reveal that multiple tyrosine phosphorylation-dependent mechanisms control cardiac myocyte K+ channel genes. Furthermore, we conclude that RPTP mu specifically regulates cardiac myocyte Kv1.5 mRNA expression. Thus this receptor protein tyrosine phosphatase may be important in responses to pathological conditions associated with the loss of cell-cell interactions in the heart.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:58:06