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Titolo:
Hyperbaric oxygen downregulates ICAM-1 expression induced by hypoxia and hypoglycemia: the role of NOS
Autore:
Buras, JA; Stahl, GL; Svoboda, KKH; Reenstra, WR;
Indirizzi:
Brigham & Womens Hosp, Dept Emergency Med, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 gency Med, Boston, MA 02115 USA Brigham & Womens Hosp, Ctr Expt Therapeut & Reperfus Injury, Dept Anesthesiol, Boston, MA 02115 USA Brigham & Womens Hosp Boston MA USA 02115 esthesiol, Boston, MA 02115 USA Boston Univ, Sch Med, Dept Pathol, Boston, MA 02118 USA Boston Univ Boston MA USA 02118 ch Med, Dept Pathol, Boston, MA 02118 USA Baylor Coll Dent, Dept Biomed Sci, Dallas, TX 75246 USA Baylor Coll Dent Dallas TX USA 75246 ept Biomed Sci, Dallas, TX 75246 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 2, volume: 278, anno: 2000,
pagine: C292 - C302
SICI:
0363-6143(200002)278:2<C292:HODIEI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; INTERCELLULAR-ADHESION MOLECULE-1; CEREBRAL-ARTERY OCCLUSION; ENDOTHELIAL-CELL-ADHESION; NITRIC-OXIDE SYNTHASE; REPERFUSION INJURY; NEUTROPHIL ADHESION; GENE-EXPRESSION; ISCHEMIA-REPERFUSION; MYOCARDIAL-ISCHEMIA;
Keywords:
cell adhesion molecules; ischemia; reperfusion injury; hyperoxia; neutrophils;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Buras, JA Brigham & Womens Hosp, Dept Emergency Med, 75 Francis St, Boston, MA 02115USA Brigham & Womens Hosp 75 Francis St Boston MA USA 02115 02115USA
Citazione:
J.A. Buras et al., "Hyperbaric oxygen downregulates ICAM-1 expression induced by hypoxia and hypoglycemia: the role of NOS", AM J P-CELL, 278(2), 2000, pp. C292-C302

Abstract

Hyperbaric oxygen (HBO) is being studied as a therapeutic intervention forischemia/reperfusion (I/R) injury. We have developed an in vitro endothelial cell model of I/R injury to study the impact of HBO on the expression ofintercellular adhesion molecule-1 (ICAM-1) and polymorphonuclear leukocyte(PMN) adhesion. Human umbilical vein endothelial cell (HUVEC) and bovine aortic endothelial cell (BAEC) induction of ICAM-1 required simultaneous exposure to both hypoxia and hypoglycemia as determined by confocal laser scanning microscopy, ELISA, and Western blot. HBO treatment reduced the expression of ICAM-1 to control levels. Adhesion of PMNs to BAECs was increased following hypoxia/hypoglycemia exposure (3.4-fold, P < 0.01) and was reduced to control levels with exposure to HBO (P = 0.67). Exposure of HUVECs and BAECs to HBO induced the synthesis of endothelial cell nitric oxide synthase(eNOS). The NOS inhibitor nitro-L-arginine methyl ester attenuated HBO-mediated inhibition of ICAM-1 expression. Our findings suggest that the beneficial effects of HBO in treating I/R injury may be mediated in part by inhibition of ICAM-1 expression through the induction of eNOS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 18:01:34