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Titolo:
Localization of cyclooxygenase-2 in human sporadic colorectal adenomas
Autore:
Chapple, KS; Cartwright, EJ; Hawcroft, G; Tisbury, A; Bonifer, C; Scott, N; Windsor, ACJ; Guillou, PJ; Markham, AF; Coletta, PL; Hull, MA;
Indirizzi:
Univ Leeds, St James Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS9 7TF S9 7TF, W Yorkshire, England Univ Leeds, St James Hosp, Dept Histopathol, Leeds LS9 7TF, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS9 7TF S9 7TF, W Yorkshire, England Univ Leeds, St James Hosp, Dept Surg, Leeds LS9 7TF, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS9 7TF S9 7TF, W Yorkshire, England
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 2, volume: 156, anno: 2000,
pagine: 545 - 553
SICI:
0002-9440(200002)156:2<545:LOCIHS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONSTEROIDAL ANTIINFLAMMATORY DRUGS; HUMAN COLON-CANCER; PROSTAGLANDIN G/H SYNTHASE-1; MESSENGER-RNA EXPRESSION; GENE-EXPRESSION; HUMAN MACROPHAGES; H SYNTHASE-2; TUMOR-GROWTH; CELL-LINES; ADENOCARCINOMAS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Chapple, KS Univ Leeds, St James Hosp, Mol Med Unit, Clin Sci Bldg, Leeds LS9 7TF, W Yorkshire, England Univ Leeds Clin Sci Bldg Leeds W Yorkshire England LS9 7TF and
Citazione:
K.S. Chapple et al., "Localization of cyclooxygenase-2 in human sporadic colorectal adenomas", AM J PATH, 156(2), 2000, pp. 545-553

Abstract

A putative target for the anti-colorectal cancer action of nonsteroidal anti-inflammatory drugs is the inducible isoform of cyclooxygenase (COX), COX-2. COX-2 is expressed within intestinal adenomas in murine polyposis models, but expression has been poorly characterized in human colorectal neoplasms. Therefore, we investigated the localization of the COX-2 protein in human sporadic colorectal adenomas, Immunohistochemistry for COX-2 and CD68 (atissue macrophage marker) was performed on formalin-fixed, paraffin-embedded (n = 52) and frozen, acetone-fixed (n = 6) sections of human sporadic colorectal adenomas, Forty of 52 (77%) formalin-fixed adenomas expressed immunoreactive COX-2. COX-2 was localized to superficial interstitial macrophages in 39 cases (75%) and to deep interstitial macrophages in 9 cases (17%). COX-2 staining of dysplastic epithelial cells was observed in 15 cases (29%). A logistic regression analysis identified the adenoma site (P = 0.012) and histological type (P = 0.001) as independent predictors of superficial macrophage COX-2 expression. There was no relationship between the number of macrophages within an adenoma and macrophage COX-2 expression. These results indicate that COX-2 is expressed predominantly by interstitial macrophages within human sporadic colorectal adenomas, If COX-2 does indeed play a role in the early stages of colorectal carcinogenesis in man, these data suggest COX-2-mediated paracrine signaling between the macrophages and epithelial cells within adenomas.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:17:14