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Titolo:
Control of apoptosis during angiogenesis by survivin expression in endothelial cells
Autore:
OConnor, DS; Schechner, JS; Adida, C; Mesri, M; Rothermel, AL; Li, FZ; Nath, AK; Pober, JS; Altieri, DC;
Indirizzi:
Yale Univ, Sch Med, Dept Dermatol, New Haven, CT 06536 USA Yale Univ New Haven CT USA 06536 , Dept Dermatol, New Haven, CT 06536 USA Yale Univ, Sch Med, Boyer Ctr Mol Med, Dept Pathol, New Haven, CT 06536 USA Yale Univ New Haven CT USA 06536 ed, Dept Pathol, New Haven, CT 06536 USA
Titolo Testata:
AMERICAN JOURNAL OF PATHOLOGY
fascicolo: 2, volume: 156, anno: 2000,
pagine: 393 - 398
SICI:
0002-9440(200002)156:2<393:COADAB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOCAL ADHESION KINASE; DEATH; REGRESSION; INDUCTION; VESSELS; DISEASE; MECHANISMS; ACTIVATION; CLEAVAGE; INJURY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Altieri, DC Yale Univ, Sch Med, Dept Dermatol, BCMM436B,295 Congress Ave, New Haven, CT 06536 USA Yale Univ BCMM436B,295 Congress Ave New Haven CT USA 06536 USA
Citazione:
D.S. O'Connor et al., "Control of apoptosis during angiogenesis by survivin expression in endothelial cells", AM J PATH, 156(2), 2000, pp. 393-398

Abstract

Mechanisms controlling endothelial cell survival during angiogenesis were investigated. Stimulation of quiescent endothelial cells with mitogens, including vascular endothelial growth factor and basic fibroblast growth factor, induced up to approximate to 16-fold up-regulation of the cell cycle-regulated apoptosis inhibitor survivin. Mitogen stimulation rapidly increased survivin RNA expression in endothelial cells, which peaked after 6 to 10 hours in culture and decreased by 24 hours. Inflammatory cytokines, tumor necrosis factor alpha, and interleukin-1 did not induce survivin expression inendothelial cells. Formation of three-dimensional vascular tubes in vitro was associated with strong induction of survivin in endothelial cells, as compared with two-dimensional cultures. By immunohistochemistry, survivin was minimally expressed in endothelium of nonproliferating capillaries of normal skin, whereas it became massively upregulated in newly formed blood vessels of granulation tissue in vivo. Recombinant expression of green fluorescent protein survivin in endothelial cells reduced caspase-3 activity and counteracted apoptosis induced by tumor necrosis factor alpha/cycloheximide,These findings identify survivin as a novel growth factor-inducible protective gene expressed by endothelial cells during angiogenesis. Therapeutic manipulation of survivin expression and function in endothelium may influence compensatory or pathological (tumor) angiogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 12:57:28