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Titolo:
INDUCTION OF DNA-DAMAGE BY RISK-FACTORS OF COLON-CANCER IN HUMAN COLON CELLS DERIVED FROM BIOPSIES
Autore:
POOLZOBEL BL; LEUCHT U;
Indirizzi:
FED RES CTR NUTR,INST NUTR PHYSIOL D-76131 KARLSRUHE GERMANY KREISKRANKENHAUS SCHWETZINGEN SCHWETZINGEN GERMANY
Titolo Testata:
Mutation research
fascicolo: 2, volume: 375, anno: 1997,
pagine: 105 - 115
SICI:
0027-5107(1997)375:2<105:IODBRO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
HETEROCYCLIC AROMATIC-AMINES; N-NITROSO COMPOUNDS; BILE-ACIDS; COLORECTAL-CANCER; IN-VITRO; 2-AMINO-1-METHYL-6-PHENYLIMIDAZO)4,5-B>PYRIDINE PHIP; EXTRAHEPATIC TISSUES; METABOLIC-ACTIVATION; F344 RATS; INVIVO;
Keywords:
COLON BIOPSY; COLON CANCER; COMET ASSAY; GENOTOXICITY; HUMAN COLON CELL; PARALLELOGRAM APPROACH; RISK FACTORS FOR COLON CARCINOGENESIS; SINGLE CELL MICROGEL ELECTROPHORESIS ASSAY; PHIP; IQ; H2O2; BENZO[A]PYRENE; LITHOCHOLIC ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
69
Recensione:
Indirizzi per estratti:
Citazione:
B.L. Poolzobel e U. Leucht, "INDUCTION OF DNA-DAMAGE BY RISK-FACTORS OF COLON-CANCER IN HUMAN COLON CELLS DERIVED FROM BIOPSIES", Mutation research, 375(2), 1997, pp. 105-115

Abstract

In order to increase the understanding of the factors responsible forcausing human colon cancer, a technique was developed to detect genotoxic effects of chemicals in human colon cells. Risk factors suspectedto be associated with the aetiology of human colon cancer were subsequently investigated: the method is based on the measurement of DNA damage in primary cells freshly isolated from human colon biopsies with the single cell microgel ectrophoresis technique ('Comet Assay'). 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-3-methyl-3H-imidazo[4,5f]quinoline (IQ), N-methyl-N-nitro-N-nitrosoguanidine (MNNG), dinitrosocaffeidine (DNC) lithocholic acid (LCA), hydrogen peroxide (H2O2) and benzo[a]pyrene (B[a]P) were investigated for their genotoxic and cytotoxic effects following 30 min incubation with colon cellsof human, and for comparative purposes also of the rat colon, The nitrosamides (MNNG, DNC) were very genotoxic in human colon cells. MNNG was more genotoxic in human than in rat colon cells. In contrast, the rat colon carcino ens PhIP and IQ were not genotoxic in human colon cells. PhIP did induce DNA damage in rat colon cells, which correlates toits capacity of inducing tumors in this animal tissue. LCA was toxic (rat > human) and concomitantly caused DNA damage in higher concentrations. The widespread contaminant B[a]P was not genotoxic in colon cells of either species using this system. H2O2 was found to be a potent genotoxic agent to both rat and human colon cells (human > rat). In summary, those compounds chosen as representatives of endogenously formedrisk factors (MNNG, H2O2, LCA) have a higher toxic and/or genotoxic potency in human colon tissue than in rat colon. They are also more effective in this system than the contaminants tested so far (B[a]P, PhIP, IQ). The newly developed technique is rapid and yields relevant results, It is a novel and useful approach to assess different chemical compounds for genotoxic activities in tumour tar et tissues of the human.

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Documento generato il 04/07/20 alle ore 13:32:45