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Titolo:
Overexpression of M68/DcR3 in human gastrointestinal tract tumors independent of gene amplification and its location in a four-gene cluster
Autore:
Bai, C; Connolly, B; Metzker, ML; Hilliard, CA; Liu, XM; Sandig, V; Soderman, A; Galloway, SM; Liu, QY; Austin, CP; Caskey, CT;
Indirizzi:
Merck Res Labs, Dept Human Genet, W Point, PA 19486 USA Merck Res Labs W Point PA USA 19486 pt Human Genet, W Point, PA 19486 USA Merck Res Labs, Dept Genet & Cellular Toxicol, W Point, PA 19486 USA MerckRes Labs W Point PA USA 19486 llular Toxicol, W Point, PA 19486 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 3, volume: 97, anno: 2000,
pagine: 1230 - 1235
SICI:
0027-8424(20000201)97:3<1230:OOMIHG>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
TNF RECEPTOR SUPERFAMILY; BREAST-CANCER; SACCHAROMYCES-CEREVISIAE; NUCLEOTIDE-SEQUENCE; RAD3 GENE; APOPTOSIS; DEATH; IDENTIFICATION; HYBRIDIZATION; CHROMOSOMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Bai, C Merck Res Labs, Dept Human Genet, WP26A, W Point, PA 19486 USA Merck Res Labs WP26A W Point PA USA 19486 6A, W Point, PA 19486 USA
Citazione:
C. Bai et al., "Overexpression of M68/DcR3 in human gastrointestinal tract tumors independent of gene amplification and its location in a four-gene cluster", P NAS US, 97(3), 2000, pp. 1230-1235

Abstract

Fas-mediated apoptosis is an important regulator of cell survival, and abnormalities in this system have been shown to result in a number of human pathological conditions. A secreted member of the tumor necrosis factor receptor superfamily, DcR3, was recently reported to be amplified in human lung and colon cancers as a negative regulator of Fas-mediated apoptosis. We identified this gene, which we call M68. M68 genomic DNA, mRNA, and protein levels were examined in a series of human gastrointestinal tract tumors. Using M68 immunohistochemistry and a scoring system similar to that used for HER-2/neu, we found that M68 protein was overexpressed in 30 of 68 (44%) human adenocarcinomas of the esophagus, stomach, colon, and rectum. Tumors examined by Northern blot revealed M68 mRNA highly elevated in a similar fraction of primary tumors from the same gastrointestinal tract regions, as well as in the colon adenocarcinoma cell lines SW480 and SW1116. Further, we found M68 protein to be overexpressed in a substantial number of tumors in which gene amplification could not be detected by fluorescence in situ hybridization or quantitative genomic PCR, suggesting that overexpression of M68 may precede amplification in tumors. Finally, we find that M68 lies within afour-gene cluster that includes a novel helicase-like gene (NHL) related to RAD3/ERCC2, a plasma membrane Ras-related GTPase and a member of the stathmin family, amplification or overexpression of which may also contribute to cell growth and tumor progression.

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Documento generato il 24/11/20 alle ore 11:14:17