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Titolo:
Confocal ratio imaging of cytoplasmic pH during germ tube growth and appressorium induction by Magnaporthe grisea
Autore:
Jelitto, TC;
Indirizzi:
Univ Edinburgh, Inst Cell & Mol Biol, Edinburgh EH9 3JH, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH9 3JH Midlothian, Scotland
Titolo Testata:
NEW PHYTOLOGIST
fascicolo: 3, volume: 144, anno: 1999,
pagine: 499 - 506
SICI:
0028-646X(199912)144:3<499:CRIOCP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLULAR ION CURRENTS; HYPHAL TIP GROWTH; CANDIDA-ALBICANS; PLASMA-MEMBRANE; ARABIDOPSIS-THALIANA; NEUROSPORA-CRASSA; INTRACELLULAR PH; POLLEN TUBES; TRANSPORT; GRADIENTS;
Keywords:
tip growth; rice blast; cytoplasmic pH; confocal ratio imaging; SNARF-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Jelitto, TC Univ Edinburgh, Inst Cell & Mol Biol, Kings Bldg, Edinburgh EH9 3JH, Midlothian, Scotland Univ Edinburgh Kings Bldg Edinburgh Midlothian Scotland EH9 3JH
Citazione:
T.C. Jelitto, "Confocal ratio imaging of cytoplasmic pH during germ tube growth and appressorium induction by Magnaporthe grisea", NEW PHYTOL, 144(3), 1999, pp. 499-506

Abstract

The role of cytosolic pH (pH(c)) in growing germ tubes of the filamentous fungus Magnaporthe grisea was analysed by confocal ratio imaging of the pH-sensitive fluorescent dye 5(6)-carboxyseminaphthorhodafluor-1 (SNARF-1). The cytosol of these cells was successfully loaded with the acetoxymethyl ester of the dye and the pH(c) was visualized and quantified during conidium germination, germ tube growth and appressorium induction by simultaneous dual-emission confocal ratio imaging. Calibrations of the free acid in vitro and calibrations in vivo produced results indicating a similar dynamic response in the pH range 6.0-8.0 for both methods. The pH(c) in growing germ tubes was consistently pH 7.2 +/- 0.1 during all developmental stages analysed. Only slight changes in pH(c) (< 0.1 pH unit) were found in response to alkaline external pH (pH 8.0). No changes in pH(c) occurred in response to anacidic extracellular pH (pH 6.0) or to the presence of nutrients. There asno observation of either pronounced gradients or changes in pH(c) in growing germ tubes accompanying conidium germination, germ tube growth or early appressorium formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 12:01:24