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Titolo:
Dose response of ethanol ingestion on antioxidant system in rat brain subcellular fractions
Autore:
Reddy, SK; Husain, K; Schlorff, EC; Scott, RB; Somani, SM;
Indirizzi:
So Illinois Univ, Sch Med, Dept Pharmacol, Springfield, IL 62794 USA So Illinois Univ Springfield IL USA 62794 acol, Springfield, IL 62794 USA
Titolo Testata:
NEUROTOXICOLOGY
fascicolo: 6, volume: 20, anno: 1999,
pagine: 977 - 987
SICI:
0161-813X(199912)20:6<977:DROEIO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPEROXIDE-DISMUTASE ACTIVITY; LIPID-PEROXIDATION; FREE-RADICALS; GLUTATHIONE-REDUCTASE; LIVER-MICROSOMES; OXIDATIVE STRESS; NERVOUS-SYSTEM; METABOLISM; CATALASE; ACETALDEHYDE;
Keywords:
ethanol; antioxidant enzyme; glutathione; lipid peroxidation; brain subcellular fraction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Somani, SM So Illinois Univ, Sch Med, Dept Pharmacol, POB 19629, Springfield, IL 62794 USA So Illinois Univ POB 19629 Springfield IL USA 62794 L 62794 USA
Citazione:
S.K. Reddy et al., "Dose response of ethanol ingestion on antioxidant system in rat brain subcellular fractions", NEUROTOXICO, 20(6), 1999, pp. 977-987

Abstract

This study investigated the response of the antioxidant defense system in brain subcellular fractions after oral graded doses of ethanol to rat. Fourgroups of male Fischer-344 rats were orally administered saline, ethanol 2g, 4 g, and 6 g/kg, respectively, and sacrificed 1 hour post treatment. Brain cytosol, synaptosomes, microsomes and mitochondria were separated by density gradient differential centrifugation and assayed for antioxidant system. A significant and dose-dependent-decrease in superoxide dismutase (SOD)activity was observed in all brain subcellular fractions. Catalase (CAT) activity was significantly decreased in brain mitochondria (67% and 80% of control) at higher doses of ethanol; whereas, CAT activity was significantlyincreased in cytosol, synaptosomes and microsomes. Glutathione peroxidase (GSH-Px) activity was significantly increased in all brain subcellular fractions except in cytosol at higher dose of ethanol. Malondialdehyde (MDA) content was significantly increased in all brain subcellular fractions showing dose response of ethanol-induced oxidative stress. The increase in MDA levels in the brain synaptosomes and microsomes were higher at 6 g dose of ethanol (155% and 163% of control) when compared to mitochondria and cytosol. Glutathione (GSH) levels were significantly increased in brain cytosol andmicrosomes at higher dose of ethanol (164% and 159% of control); whereas, the GSH concentration was significantly decreased in brain synaptosomes andmitochondria. The antioxidant enzyme (AOE) activity ratios (GSH-Px/SOD andGSH-Pr + CAT/SOD) were dose dependently increased in all brain subcellularfractions, particularly in synaptosomes. The GSH/GSSG ratio was dose dependently increased in brain microsomes. The perturbations in the antioxidant defense system and enhanced lipid peroxidation following graded doses of ethanol ingestion indicate a dose-dependent-oxidative 2133stress response in brain subcellular compartments of rats. (C) 1999 Intox Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 06:15:29