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Titolo:
Interactions of bovine brain tubulin with pyridostigmine bromide and N,N '-diethyl-m-toluamide
Autore:
Prasad, V; Scotch, R; Chaudhuri, AR; Walss, C; Fathy, DB; Miller, C; Luduena, RF;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Biochem, San Antonio, TX 78284 USA Univ Texas San Antonio TX USA 78284 pt Biochem, San Antonio, TX 78284 USA Univ Texas, Hlth Sci Ctr, Dept Family Practice, San Antonio, TX 78284 USA Univ Texas San Antonio TX USA 78284 y Practice, San Antonio, TX 78284 USA
Titolo Testata:
NEUROCHEMICAL RESEARCH
fascicolo: 1, volume: 25, anno: 2000,
pagine: 19 - 25
SICI:
0364-3190(200001)25:1<19:IOBBTW>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED DELAYED NEUROTOXICITY; N,N-DIETHYL-M-TOLUAMIDE DEET; CELL-PROLIFERATION; DERMAL APPLICATION; INSECT REPELLENTS; ABSORPTION; EXPOSURE; VINBLASTINE; INHIBITION; MECHANISM;
Keywords:
pyridostigmine bromide; pyridostigmine; N,N '-diethyl-m-toluamide; microtubule; tubulin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Luduena, RF Univ Texas, Hlth Sci Ctr, Dept Biochem, San Antonio, TX 78284 USA Univ Texas San Antonio TX USA 78284 San Antonio, TX 78284 USA
Citazione:
V. Prasad et al., "Interactions of bovine brain tubulin with pyridostigmine bromide and N,N '-diethyl-m-toluamide", NEUROCHEM R, 25(1), 2000, pp. 19-25

Abstract

Pyridostigmine bromide (PB), an inhibitor of acetylcholinesterase, has been used as a prophylactic for nerve gas poisoning. N,N'-diethyl-m-toluamide (DEET) is the active ingredient in most insect repellents and is thought tointeract synergistically with PB. Since PB can inhibit the binding of organophosphates to tubulin and since organophosphates inhibit microtubule assembly, we decided to examine the effects of PB and DEET on microtubule assembly as well as their interactions with tubulin, the subunit protein of microtubules. We found that PB binds to tubulin with an apparent K-d of about 60 mu M. PB also inhibits microtubule assembly in vitro, although at higher concentrations PB induces formation of tubulin aggregates of high absorbance. Like PB, DEET is a weak inhibitor of microtubule assembly and also induces formation of tubulin aggregates. Many tubulin ligands stabilize the conformation of tubulin as measured by exposure of sulfhydryl groups and hydrophobic areas and stabilization of colchicine binding. PB appears to have very little effect on tubulin conformation, and DEET appears to have no effect. Neither compound interferes with colchicine binding to tubulin. Our results raise the possibility that PB and DEET may exert some of their effects in vivo by interfering with microtubule assembly or function, although high intracellular levels of these compounds would be required.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:33:37