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Titolo:
Expression of cadherin adhesion molecules on human gametes
Autore:
Rufas, O; Fisch, B; Ziv, S; Shalgi, R;
Indirizzi:
Tel Aviv Univ, Sackler Sch Med, Dept Embryol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 mbryol, IL-69978 Tel Aviv, Israel Tel Aviv Univ, Rabin Med Ctr, Dept Obstet & Gynecol, IL-69978 Tel Aviv, Israel Tel Aviv Univ Tel Aviv Israel IL-69978 ynecol, IL-69978 Tel Aviv, Israel
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 2, volume: 6, anno: 2000,
pagine: 163 - 169
SICI:
1360-9947(200002)6:2<163:EOCAMO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN EGG ACTIVATION; CELL CELL-ADHESION; N-CADHERIN; MOUSE; PREIMPLANTATION; UVOMORULIN; EMBRYOS; KINASE; FUSION; SPERM;
Keywords:
cadherins; gamete fusion; oocytes; spermatozoa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Shalgi, R Tel Aviv Univ, Sackler Sch Med, Dept Embryol, Campus Beilinson, IL-69978 Tel Aviv, Israel Tel Aviv Univ Campus Beilinson Tel Aviv Israel IL-69978 Israel
Citazione:
O. Rufas et al., "Expression of cadherin adhesion molecules on human gametes", MOL HUM REP, 6(2), 2000, pp. 163-169

Abstract

The presence of cadherins, Ca2+-dependent cell-cell adhesion molecules which may be involved in gamete interaction, was investigated in human gametes. Expression of cadherin molecules was demonstrated using an anti-pan-cadherin antibody and specific: antibodies against the three classical cadherins: E- (epithelial), P- (placental) and N- (neural) cadherins. Samples of 48 h old unfertilized oocytes and spermatozoa from in-vitro fertilizing semen samples were lysed and separated by electrophoresis. Localization of cadherins was determined on intact, fixed, permeabilized spermatozoa and oocytes by immunocytochemistry assessed by confocal microscopy. Immunoblotting withthe pan-cadherin antibody revealed a single band of similar to 120 kDa in spermatozoa (whether 'fresh', capacitated, or frozen-thawed) and oocyte extracts. Oocytes presented all three classical cadherins with the appropriatemolecular weights of 120-130 kDa. In sperm lysate we demonstrated the presence of E-cadherin but not N-cadherin. The anti-P antibody detected a 90 kDa peptide as the only immunoreactive antigen. Following immunocytochemistryof human oocytes all cadherin molecules were allocated predominantly to the plasma membrane with only traces in the cytoplasm. In spermatozoa, several staining patterns were observed with each of the pan-cadherin, N-cadherinand E-cadherin antibodies mostly confined to different head regions. We conclude that cadherin molecules are present on plasma membranes of both human spermatozoa and oocytes and may play a role in the intricate recognition process preceding gamete fusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 16:13:32