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Titolo:
PRESENTATION OF A FOREIGN PEPTIDE ON THE SURFACE OF TOMATO BUSHY STUNT VIRUS
Autore:
JOELSON T; AKERBLOM L; OXELFELT P; STRANDBERG B; TOMENIUS K; MORRIS TJ;
Indirizzi:
UPPSALA UNIV,CTR BIOMED,DEPT BIOL MOL,POB 590 S-75124 UPPSALA SWEDEN NATL VET INST,DEPT VIROL,CTR BIOMED S-75123 UPPSALA SWEDEN SWEDISH UNIV AGR SCI,DEPT PLANT PATHOL S-75007 UPPSALA SWEDEN UNIV NEBRASKA,SCH BIOL SCI LINCOLN NE 68588
Titolo Testata:
Journal of General Virology
, volume: 78, anno: 1997,
parte:, 6
pagine: 1213 - 1217
SICI:
0022-1317(1997)78:<1213:POAFPO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT-VIRUS; MOSAIC-VIRUS; EXPRESSION; PARTICLE; RESOLUTION; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
T. Joelson et al., "PRESENTATION OF A FOREIGN PEPTIDE ON THE SURFACE OF TOMATO BUSHY STUNT VIRUS", Journal of General Virology, 78, 1997, pp. 1213-1217

Abstract

A 13-amino-acid peptide derived from the V3 loop of human immunodeficiency virus (HIV-1) glycoprotein 120 (gp120) was attached as a C-terminal gene fusion to the coat protein of tomato bushy stunt virus (TBSV). The architecture of this plant virus permitted external display of the foreign sequence 180 times on the surface of the chimaeric virus particle. The chimaera replicated to a level similar to wild-type TBSV and the foreign sequence was retained through six sequential passages in plants. The HIV epitope was detected on the surface of the virus capsid by a VS-specific monoclonal antibody and by human sera from HIV-1-positive patients, demonstrating the potential of using plant-derived chimaeric particles for diagnostic purposes. Chimaeric virus also induced a specific immune response to the foreign HIV epitope when injected into NMRI mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:36:08