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Titolo:
Absence of CD89, polymeric immunoglobulin receptor, and asialoglycoproteinreceptor on human mesangial cells
Autore:
Leung, JCK; Tsang, AWL; Chan, DTM; Lai, KN;
Indirizzi:
Univ Hong Kong, Queen Mary Hosp, Dept Med, Pokfulam, Hong Kong Univ Hong Kong Pokfulam Hong Kong y Hosp, Dept Med, Pokfulam, Hong Kong
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 2, volume: 11, anno: 2000,
pagine: 241 - 249
SICI:
1046-6673(200002)11:2<241:AOCPIR>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-ALPHA RECEPTORS; IGA NEPHROPATHY; SECRETORY COMPONENT; EPITHELIAL TRANSPORT; R EXPRESSION; BINDING; BLOOD; DISEASE; CHARGE; KIDNEY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Lai, KN Univ Hong Kong, Queen Mary Hosp, Dept Med, Pokfulam, Hong Kong Univ Hong Kong Pokfulam Hong Kong Dept Med, Pokfulam, Hong Kong
Citazione:
J.C.K. Leung et al., "Absence of CD89, polymeric immunoglobulin receptor, and asialoglycoproteinreceptor on human mesangial cells", J AM S NEPH, 11(2), 2000, pp. 241-249

Abstract

IgA nephropathy (IgAN) is characterized by raised serum IgA and predominant mesangial IgA deposits of polymeric nature. The expression of IgA receptor molecules in white blood cells and glomerular mesangial cells has recently attracted much attention in relation to the uptake of IgA by these cells. This study investigates the expression of IgA Fc receptor (Fc alpha R1 or CD89), asialoglycoprotein receptor (ASGPR), and polymeric Ig receptor (pIgR) in cultured glomerular mesangial cells. Using a sensitive nested reverse transcription-PCR, mRNA encoding for Fc alpha R1, pIgR, or the H2 chain of ASGPR was not demonstrated on human mesangial cells. U937, HepG2, and HT29 cell lines, used as positive controls, strongly expressed the FcaR1, ASGPR,and pIgR mRNA, respectively, under similar experimental conditions. Flow cytometry also demonstrated the presence of surface proteins for Fc alpha R1, ASGPR, and pIgR on the respective control cell lines but not on human mesangial cells. Expression of Fc alpha R1 mRNA on cultured U937 cells was upregulated by tumor necrosis factor-alpha. However, tumor necrosis factor-alpha, interleukin-1 beta, or transforming growth factor-beta failed to inducethe expression of FcaR1 on human mesangial cells. Human serum IgA or secretory IgA bound to human mesangial cells, HepG2, or the U937 cell line in a dose-dependent manner. The binding of purified IgA to human mesangial cellswas not blocked by preincubation with human IgG, IgM, orosomucoid, asialoorosomucoid, anti-CD89 antibody (My43), or anti-secretory component antibody. The present study concluded that there was an absence of Fc alpha R1, ASGPR, or pIgR on human mesangial cells. These findings suggest that the predominant binding of human IgA to human mesangial cells is mediated by other mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 00:06:06