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Titolo:
Comparison of cysteine string protein (Csp) and mutant alpha-SNAP overexpression reveals a role for Csp in late steps of membrane fusion in dense-core granule exocytosis in adrenal chromaffin cells
Autore:
Graham, ME; Burgoyne, RD;
Indirizzi:
Univ Liverpool, Physiol Lab, Liverpool L69 3BX, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3BX , Merseyside, England
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 4, volume: 20, anno: 2000,
pagine: 1281 - 1289
SICI:
0270-6474(20000215)20:4<1281:COCSP(>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ETHYLMALEIMIDE-SENSITIVE FACTOR; REGULATED EXOCYTOSIS; NEUROTRANSMITTER RELEASE; SYNAPTIC EXOCYTOSIS; MEDULLARY CELLS; ATPASE ACTIVITY; IN-VITRO; T-SNARE; COMPLEX; DROSOPHILA;
Keywords:
exocytosis; secretion; SNAP; NSF; Csp; amperometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Burgoyne, RD Univ Liverpool, Physiol Lab, Crown St, Liverpool L69 3BX, Merseyside, England Univ Liverpool Crown St Liverpool Merseyside England L69 3BX
Citazione:
M.E. Graham e R.D. Burgoyne, "Comparison of cysteine string protein (Csp) and mutant alpha-SNAP overexpression reveals a role for Csp in late steps of membrane fusion in dense-core granule exocytosis in adrenal chromaffin cells", J NEUROSC, 20(4), 2000, pp. 1281-1289

Abstract

Assembly of the SNARE complex and its disassembly caused by the action of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) and NSF is crucial for the maintenance of vesicular traffic, including fusion of regulated exocytotic vesicles. Various other proteins may also have important roles in the processes leading to membrane fusion via interaction with the SNARE proteins, including the secretory vesicle cysteine string protein (Csp). Here we have examined the effect of overexpression of a dominant negative alpha-SNAP mutant or Csp on exocytosis of dense-core granules insingle chromaffin cells monitored using amperometry to detect released catecholamine. Exocytosis of trans-Golgi network (TGN)-derived dense-core granules was substantially inhibited by expression of alpha-SNAP(L294A). The amplitude and characteristics of the individual release events were unaffected by expression of alpha-SNAP(L294A), consistent with an essential role foralpha-SNAP in early steps of priming but not in the fusion process. In contrast, Csp overexpression, which also inhibited the extent of exocytosis, also modified the kinetics of the individual release events seen as an increase in the rise time and a broadening of the residual amperometric spikes in Csp-transfected cells. These results suggest that unlike alpha-SNAP, Csp plays a key role in the protein interactions close to the fusion process orfusion pore opening during Ca2+-regulated exocytosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 12:42:31