Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Syndecan-1 shedding is enhanced by LasA, a secreted virulence factor of Pseudomonas aeruginosa
Autore:
Park, PW; Pier, GB; Preston, MJ; Goldberger, O; Fitzgerald, ML; Bernfield, M;
Indirizzi:
Harvard Univ, Childrens Hosp, Sch Med, Dept Med,Div Newborn Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ed,Div Newborn Med, Boston, MA 02115 USA Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med,Channing Lab, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 t Med,Channing Lab, Boston, MA 02115 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 5, volume: 275, anno: 2000,
pagine: 3057 - 3064
SICI:
0021-9258(20000204)275:5<3057:SSIEBL>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPARAN-SULFATE PROTEOGLYCANS; NECROSIS-FACTOR RECEPTOR; CELL-SURFACE; METALLOPROTEASE INHIBITOR; EPITHELIAL-CELLS; FACTOR-ALPHA; L-SELECTIN; BACTERIAL PROTEINASES; BORDETELLA-PERTUSSIS; MAMMALIAN-CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Bernfield, M Harvard Univ, Childrens Hosp, Sch Med, Dept Med,Div Newborn Med, 300 Longwood Ave,Enders 9, Boston, MA 02115 USA Harvard Univ 300 Longwood Ave,Enders 9 Boston MA USA 02115 SA
Citazione:
P.W. Park et al., "Syndecan-1 shedding is enhanced by LasA, a secreted virulence factor of Pseudomonas aeruginosa", J BIOL CHEM, 275(5), 2000, pp. 3057-3064

Abstract

Microbial pathogens frequently take advantage of host systems for their pathogenesis. Shedding of cell surface molecules as soluble extracellular domains (ectodomains) is one of the host responses activated during tissue injury. In this study, we examined whether pathogenic bacteria can modulate shedding of syndecan-1, the predominant syndecan of host epithelia. Our studies found that overnight culture supernatants of Pseudomonas aeruginosa and Staphylococcus aureus enhanced the shedding of syndecan-1 ectodomains, whereas culture supernatants of several other Gramnegative and Gram-positive bacteria had only low levels of activity. Because supernatants from all tested strains of P, aeruginosa (n = 9) enhanced syndecan-1 shedding by more than 4-fold above control levels, we focused our attention on this Gram-negative bacterium. Culture supernatants of P. aeruginosa increased shedding of syndecan-1 in both a concentration- and time-dependent manner, and augmentedshedding by various host cells. A 20-kDa shedding enhancer was partially purified from the supernatant through ammonium sulfate precipitation and gelchromatography, and identified by N-terminal sequencing as LasA, a known P. aeruginosa virulence factor. LasA was subsequently determined to be a syndecan-1 shedding enhancer from the findings that (i) immunodepletion of LasA from the partially purified sample resulted in abrogation of its activityto enhance shedding and (ii) purified LasA increased shedding in a concentration-dependent manner. Our results also indicated that LasA enhances syndecan-1 shedding by activation of the host cell's shedding mechanism and notby direct interaction with syndecan-1 ectodomains, Enhanced syndecan-1 shedding may be a means by which pathogenic bacteria take advantage of a host mechanism to promote their pathogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 22:38:00