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Titolo:
Role of pinoline and melatonin in stabilizing hepatic microsomal membranesagainst oxidative stress
Autore:
Garcia, JJ; Reiter, RJ; Pie, J; Ortiz, GG; Cabrera, J; Sainz, RM; Acuna-Castroviejo, D;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 truct Biol, San Antonio, TX 78229 USA Univ Zaragoza, Dept Physiol & Pharmacol, E-50009 Zaragoza, Spain Univ Zaragoza Zaragoza Spain E-50009 Pharmacol, E-50009 Zaragoza, Spain Univ Granada, Dept Physiol, E-18071 Granada, Spain Univ Granada Granada Spain E-18071 Dept Physiol, E-18071 Granada, Spain
Titolo Testata:
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
fascicolo: 6, volume: 31, anno: 1999,
pagine: 609 - 616
SICI:
0145-479X(199912)31:6<609:ROPAMI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPID-PEROXIDATION PRODUCTS; IN-VITRO; NEUROHORMONE MELATONIN; ANTIOXIDANT ACTIVITY; RADICAL SCAVENGER; ALPHA-TOCOPHEROL; BETA-CARBOLINES; VITAMIN-E; FLUIDITY; DAMAGE;
Keywords:
pinoline; melatonin; lipid peroxidation; membrane fluidity; microsome;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Reiter, RJ Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, 7703 Floyd Curl Dr,San Antonio, TX 78229 USA Univ Texas 7703 Floyd Curl Dr San Antonio TX USA 78229 8229 USA
Citazione:
J.J. Garcia et al., "Role of pinoline and melatonin in stabilizing hepatic microsomal membranesagainst oxidative stress", J BIOENER B, 31(6), 1999, pp. 609-616

Abstract

We investigated the influence of pinoline (0.01-1.5 mM) on microsomal membrane fluidity before and after rigidity was induced by oxidative stress. Inaddition, we tested the effect of pinoline in the presence of 1 mM melatonin. The fluidity in rat hepatic microsomes was monitored using fluorescencespectroscopy and it was compared to the inhibition of malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) production as a reflection of lipid peroxidation. Below 0.6 mM, pinoline inhibited membrane rigidity in a manner parallel to its inhibitory effect on MDA + 4-HDA formation. At concentrations between 1-1.5 mM, pinoline was less effective in stabilizing microsomal membranes than was predicted from its inhibition of lipid peroxidation. The addition of 1 mM melatonin enhanced the membrane-stabilizing activity of pinoline (0.01-0.6 mM). This cooperative effect was not observed for concentrations of pinoline between 1-1.5 mM. When pinoline was tested without induced oxidative damage, 1-1.5 mM pinoline maintained membrane fluidity at the same level as that recorded after induced lipid peroxidation. The results suggest that pinoline may be another pineal molecule that prevents membrane rigidity mediated by lipid peroxidation and this ability is enhanced by melatonin.

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Documento generato il 25/09/20 alle ore 00:06:11