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Titolo:
Regulation of human and mouse oocyte maturation in vitro with 6-dimethylaminopurine
Autore:
Anderiesz, C; Fong, CY; Bongso, A; Trounson, AO;
Indirizzi:
Monash Univ, Ctr Early Human Dev, Inst Reprod & Dev, Monash Med Ctr, Clayton, Vic 3168, Australia Monash Univ Clayton Vic Australia 3168 Ctr, Clayton, Vic 3168, Australia Natl Univ Singapore, Dept Obstet & Gynaecol, Singapore 0511, Singapore Natl Univ Singapore Singapore Singapore 0511 , Singapore 0511, Singapore
Titolo Testata:
HUMAN REPRODUCTION
fascicolo: 2, volume: 15, anno: 2000,
pagine: 379 - 388
SICI:
0268-1161(200002)15:2<379:ROHAMO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERMINAL VESICLE BREAKDOWN; PROTEIN-SYNTHESIS INHIBITORS; HISTONE-H1 KINASE-ACTIVITY; CELL-CYCLE CONTROL; MEIOTIC MATURATION; BOVINE OOCYTES; PROMOTING FACTOR; PORCINE OOCYTES; SHEEP OOCYTES; PIG OOCYTES;
Keywords:
6-dimethylaminopurine; histone H1 kinase; human oocytes; in-vitro maturation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Anderiesz, C Monash Univ, Ctr Early Human Dev, Inst Reprod & Dev, Monash Med Ctr, Level5,246 Clayton Rd, Clayton, Vic 3168, Australia Monash Univ Level 5,246 Clayton Rd Clayton Vic Australia 3168
Citazione:
C. Anderiesz et al., "Regulation of human and mouse oocyte maturation in vitro with 6-dimethylaminopurine", HUM REPR, 15(2), 2000, pp. 379-388

Abstract

It has been postulated that premature shortening of the oocyte growth phase due to the recovery of oocytes from small diameter follicles may be responsible for the developmental anomalies associated with in-vitro maturation.6-Dimethylaminopurine (DMAP) was used to artificially lengthen the pre-maturation period of oocyte growth, in vitro, by inhibiting germinal vesicle breakdown in mouse and human oocytes. DMAP inhibited the meiotic maturation of mouse and human oocytes and the inhibition was fully reversible, The timing of polar body extrusion was accelerated in mouse oocytes following the withdrawal of DMAP; however, the kinetics of nuclear maturation in human oocytes was unaffected by exposure to DMAP, All. mouse and human DMAP-treatedoocytes that matured to metaphase II expressed histone H1 kinase activity. Fertilization rates in both DMAP-treated and control mouse and human oocytes were comparable, and human embryonic development was similar in control and DMAP-treated oocytes. However, blastocyst development was significantlyreduced in DMAP-treated mouse oocytes (P < 0.05), It is concluded that lengthening the pre-maturation growth phase, by temporarily inhibiting kinase activity with DMAP, does not directly improve oocyte developmental competence but provides a useful tool for further investigating meiotic and developmentally related events in vitro by manipulating meiotic resumption.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 07:42:09