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Titolo:
Cloning and characterization of cDNA encoding Cdc2 kinase, a component of maturation-promoting factor, in Rana dybowshii
Autore:
Bandyopadhyay, J; Bandyopadhyay, A; Choi, HS; Kwon, HB; Kang, HM;
Indirizzi:
Chongju Univ, Dept Genet Engn, Sangdang Gu, Chongju 360764, Chungbuk, South Korea Chongju Univ Chongju Chungbuk South Korea 360764 4, Chungbuk, South Korea Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea
Titolo Testata:
GENERAL AND COMPARATIVE ENDOCRINOLOGY
fascicolo: 2, volume: 117, anno: 2000,
pagine: 313 - 322
SICI:
0016-6480(200002)117:2<313:CACOCE>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEIOTIC MATURATION; OOCYTE MATURATION; XENOPUS OOCYTES; PROTEIN-KINASE; CELL-CYCLE; MOLECULAR MECHANISMS; CYTOPLASMIC FACTOR; MAP KINASE; M-PHASE; MPF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kang, HM Chongju Univ, Dept Genet Engn, Sangdang Gu, Chongju 360764, Chungbuk, South Korea Chongju Univ Chongju Chungbuk South Korea 360764 uk, South Korea
Citazione:
J. Bandyopadhyay et al., "Cloning and characterization of cDNA encoding Cdc2 kinase, a component of maturation-promoting factor, in Rana dybowshii", GEN C ENDOC, 117(2), 2000, pp. 313-322

Abstract

In order to understand the mechanism of oocyte maturation in seasonal-breeding wild frogs, we have cloned and sequenced a cDNA encoding Cdc2 kinase, a component of the maturation-promoting factor (MPF) in Rana dybowskii. About 1.2-kb cDNA was isolated by reverse transcription coupled to polymerase chain reaction (RT-PCR) and cDNA library screening. The cloned Rana Cdc2 cDNA encodes a complete open-reading frame with 302 amino acid residues, which deduce a 34-kDa protein. Homology of more than 80% was found between the deduced amino acid sequence of Rana Cdc2 and that of five phylogenetically distant organisms, and 94% identity was found between Rana and Xenopus. More importantly, the Thrl4, Tyrl5, and Thr161 residues, the phosphorylation sites for the activation of the enzyme, are highly conserved. In vitro-translated Rana Cdc2 cross-reacted with Xenopus p34(cdc2) antibody as shown by Western blot. Northern blot analysis showed that a 1.7-kb transcript was highly expressed in the gonads compared to other tissues, indicating the important role of Cdc2 kinase in gonads as a component of MPF The cloned Rana Cdc2 cDNA also exhibited histone H1 kinase activity when expressed in CV-1 cells. In the present study, therefore, we have characterized the Rana Cdc2 kinase in amphibian, which will be helpful in understanding the process of oocyte maturation related to the reproduction cycle of wild frogs. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 23:42:32