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Titolo:
Characterization of the c-specific promoter of the gene encoding human endothelin-converting enzyme-1 (ECE-1)
Autore:
Funke-Kaiser, H; Bolbrinker, J; Theis, S; Lemmer, J; Richter, CM; Paul, M; Orzechowski, HD;
Indirizzi:
Free Univ Berlin, Benjamin Franklin Med Ctr, Inst Clin Pharmacol & Toxicol, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 Toxicol, D-12200 Berlin, Germany
Titolo Testata:
FEBS LETTERS
fascicolo: 2-3, volume: 466, anno: 2000,
pagine: 310 - 316
SICI:
0014-5793(20000128)466:2-3<310:COTCPO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPING AVIAN HEART; TRANSCRIPTION FACTOR; ALTERNATIVE PROMOTERS; MESSENGER-RNA; EXPRESSION; CELLS; ISOFORMS; PROTEIN; GATA; METALLOPROTEASE;
Keywords:
endothelin-converting enzyme 1; alternative promoter; ETS; GATA; E2F; initiator element (Inr);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Paul, M Free Univ Berlin, Benjamin Franklin Med Ctr, Inst Clin Pharmacol &Toxicol, Hindenburgdamm 30, D-12200 Berlin, Germany Free Univ Berlin Hindenburgdamm 30 Berlin Germany D-12200 Germany
Citazione:
H. Funke-Kaiser et al., "Characterization of the c-specific promoter of the gene encoding human endothelin-converting enzyme-1 (ECE-1)", FEBS LETTER, 466(2-3), 2000, pp. 310-316

Abstract

Human ECE-1 is expressed in four isoforms with different tissue distribution and its mRNA and protein levels are altered under certain pathophysiological conditions. To investigate the transcriptional regulation of ECE-1, westudied the regulatory region of ECE-1c, the major ECE-1 isoform, A genomic clone comprising the complete human ECE-1 gene including the putative ECE-1c-specific promoter was obtained, Up to 968 bp upstream of the putative c-specific translation initiation start codon and several serial deletion mutants were subcloned into a reporter vector and transfected into endothelial (BAEC, EA.hy926, ECV304) and epithelial (MDA MB435S, MCF7) cells, showingvery strong promoter activity in comparison to the SV40 promoter and to the previously described ECE-1a and 1b promoters. Transfection of serial deletion mutants indicated two positive regulatory regions within the promoter (-142/-240 and -240/490) likely involved in binding GATA and ETS transcription factors, RNase protection assay (RPA) and 5'-RACE revealed multiple transcriptional start sites located at about -110, -140 and -350 bp, Site-directed mutagenesis; demonstrated a crucial role for the E2F cis-element for basal ECE-1c promoter activity, Additionally, we found a correlation betweenisoform-specific ECE-1 mRNA levels and corresponding ECE-1a, 1b, 1c promoter activities. (C) 2000 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 08:59:20