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Titolo:
p97 ATPase, an ATPase involved in membrane fusion, interacts with DNA unwinding factor (DUF) that functions in DNA replication
Autore:
Yamada, T; Okuhara, K; Iwamatsu, A; Seo, H; Ohta, K; Shibata, T; Murofushi, H;
Indirizzi:
RIKEN, Cellular & Mol Biol Lab, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 l Biol Lab, Wako, Saitama 3510198, Japan Univ Tokyo, Fac Sci, Dept Biophys & Biochem, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Biochem, Bunkyo Ku, Tokyo 1130033, Japan Kirin Brewery Co Ltd, Cent Labs Key Technol, Sect Prot Chem, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Kirin Brewery Co Ltd Yokohama Kanagawa Japan 2360004 agawa 2360004, Japan
Titolo Testata:
FEBS LETTERS
fascicolo: 2-3, volume: 466, anno: 2000,
pagine: 287 - 291
SICI:
0014-5793(20000128)466:2-3<287:PAAAII>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE; SACCHAROMYCES-CEREVISIAE; POLYMERASE-ALPHA; PROTEIN; CDC48P; BINDING; GENE; VCP; NSF;
Keywords:
DNA replication; DNA unwinding factor; p97 ATPase; Xenopus egg extract;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Yamada, T RIKEN, Cellular & Mol Biol Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan RIKEN 2-1 Hirosawa Wako Saitama Japan 3510198 ama 3510198, Japan
Citazione:
T. Yamada et al., "p97 ATPase, an ATPase involved in membrane fusion, interacts with DNA unwinding factor (DUF) that functions in DNA replication", FEBS LETTER, 466(2-3), 2000, pp. 287-291

Abstract

DNA unwinding factor (DUF) unwinds duplex DNA and is supposed to function in DNA replication in Xenopus egg extracts, Here we report the isolation and analysis of a DUF-interacting factor. By immunoprecipitation, we found that p97 ATPase (p97) interacts with DUF in Xenopus egg extracts. This interaction was confirmed by the in vitro binding of purified p97 with DUF, When sperm chromatin was added to Xenopus egg extracts to construct nuclei active in DNA replication, p97 was incorporated into the nuclei. These data suggest that the complex of DUF and p97 may function in DNA replication. (C) 2000 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:24:53