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Titolo:
Capillary electrophoresis with end-capillary potentiometric detection using a copper electrode
Autore:
Macka, M; Gerhardt, G; Andersson, P; Bogan, D; Cassidy, RM; Haddad, PR;
Indirizzi:
Univ Tasmania, Fac Sci & Engn, Sch Chem, Separat Sci Grp, Hobart, Tas 7001, Australia Univ Tasmania Hobart Tas Australia 7001 Grp, Hobart, Tas 7001, Australia Univ Saskatchewan, Dept Chem, Saskatoon, SK, Canada Univ Saskatchewan Saskatoon SK Canada , Dept Chem, Saskatoon, SK, Canada
Titolo Testata:
ELECTROPHORESIS
fascicolo: 12, volume: 20, anno: 1999,
pagine: 2539 - 2546
SICI:
0173-0835(199908)20:12<2539:CEWEPD>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ION CHROMATOGRAPHY; INORGANIC ANIONS; ZONE ELECTROPHORESIS; ORGANIC-ACIDS; CATIONS;
Keywords:
capillary electrophoresis; potentiometric detection; end-capillary detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Haddad, PR Univ Tasmania, Fac Sci & Engn, Sch Chem, Separat Sci Grp, GPO Box 252-75, Hobart, Tas 7001, Australia Univ Tasmania GPO Box 252-75 Hobart Tas Australia 7001 ustralia
Citazione:
M. Macka et al., "Capillary electrophoresis with end-capillary potentiometric detection using a copper electrode", ELECTROPHOR, 20(12), 1999, pp. 2539-2546

Abstract

Potentiometric end-capillary detection in capillary electrophoresis has the advantage of relatively easy miniaturisation without having to compromisethe concentration sensitivity. Potentiometric end-capillary detection using a copper electrode is also attractive because of the sensitive detection of many inorganic and organic UV-transparent ions and the ability to work in both direct and indirect mode. In this work, detection of a number of common anions in a tartrate electrolyte at pH 3 was studied. The influence of the end-capillary detection geometry on the detection performance was investigated. An end-capillary detection cell allowing the separation capillary to be changed without the need to realign the detection electrode was constructed and fitted into a commercial CE apparatus. Under the optimal configuration, which was a 25 mu m diameter copper electrode aligned coaxially with a 25 mu m capillary and positioned at a distance of about 25 mu m from the capillary end, excellent peak shapes were achieved and comparison with simultaneous on-capillary photometric detection showed no additional peak broadening. Good sensitivity was obtained, resulting in concentration limits of detection (LODs) in the low mu M range and mass LODs in the low amor range. Examples of separations of inorganic and organic anions are presented and the analytical potential of the detection method is assessed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:01:05