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Titolo:
Glycine betaine, carnitine, and choline enhance salinity tolerance and prevent the accumulation of sodium to a level inhibiting growth of Tetragenococcus halophila
Autore:
Robert, H; Le Marrec, C; Blanco, C; Jebbar, M;
Indirizzi:
Univ Rennes 1, CNRS, UPRESA 6026, Grp Membranes & Osmoregulat, F-35042 Rennes, France Univ Rennes 1 Rennes France F-35042 Osmoregulat, F-35042 Rennes, France Univ Bordeaux 1, ENSSTAB, Lab Microbiol Alimentaire & Biotechnol, F-33405 Talence, France Univ Bordeaux 1 Talence France F-33405 otechnol, F-33405 Talence, France COBIOTEX, F-53960 Bonchamp Les Laval, France COBIOTEX Bonchamp Les LavalFrance F-53960 60 Bonchamp Les Laval, France
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 2, volume: 66, anno: 2000,
pagine: 509 - 517
SICI:
0099-2240(200002)66:2<509:GBCACE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACTOBACILLUS-PLANTARUM; COMPATIBLE SOLUTES; BREVIBACTERIUM LINENS; BACILLUS-SUBTILIS; TRANSPORT-SYSTEM; ESCHERICHIA-COLI; STRESS; OSMOREGULATION; PEDIOCOCCUS; ADAPTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Jebbar, M Univ Rennes 1, CNRS, UPRESA 6026, Grp Membranes & Osmoregulat, Campus Beaulieu, F-35042 Rennes, France Univ Rennes 1 Campus Beaulieu Rennes France F-35042 nes, France
Citazione:
H. Robert et al., "Glycine betaine, carnitine, and choline enhance salinity tolerance and prevent the accumulation of sodium to a level inhibiting growth of Tetragenococcus halophila", APPL ENVIR, 66(2), 2000, pp. 509-517

Abstract

Natural-abundance C-13-nuclear magnetic resonance was used to probe the intracellular organic solute content of the moderately halophilic bacterium Tetragenococcus halophila. When grown in complex growth media supplemented or not with NaCl, T. halophila accumulates glycine betaine and carnitine. Unlike other moderate halophiles, T. halophila was not able to produce potentosmoprotectants (such as ectoines and glycine betaine) through de novo synthesis when cultured in defined medium under hyperosmotic constraint. Addition of 2 mM carnitine, glycine betaine, or choline to defined medium improved growth parameters, not only at high salinity (up to 2.5 M NaCl) but alsoin media lacking NaCl. These compounds were taken up when available in thesurrounding medium. The transport activity occurred at low and high salinities and seems to be constitutive. Glycine betaine and carnitine were accumulated by T. halophila in an unmodified form, while exogenously provided choline led to an intracellular accumulation of glycine betaine. This is the first evidence of the existence of a choline-glycine betaine pathway in a lactic acid bacterium. An assay showed that the compatible solutes strikingly repressed the accumulation of glutamate and slightly increased the intracellular potassium level only at high salinity. Interestingly, osmoprotectant-treated cells were able to maintain the intracellular sodium concentration at a relatively constant Level (200 to 300 nmol/mg [dry weight]), independent of the NaCl concentration of the medium. In contrast, in the absence of osmoprotectant, the intracellular sodium content increased sharply from 200 to 2,060 nmol/mg (dry weight) when the salinity of the medium was raisedfrom 1 to 2 M. Indeed, the imported compatible solutes play an actual rolein regulating the intracellular Na+ content and confer a much higher salt tolerance to T. halophila.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 18:18:44