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Titolo:
Interspecific transfer of Streptomyces giant linear plasmids in sterile amended soil microcosms
Autore:
Ravel, J; Wellington, EMH; Hill, RT;
Indirizzi:
Univ Maryland, Inst Biotechnol, Ctr Marine Biotechnol, Baltimore, MD 21202USA Univ Maryland Baltimore MD USA 21202 e Biotechnol, Baltimore, MD 21202USA Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL 7AL, W Midlands, England
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 2, volume: 66, anno: 2000,
pagine: 529 - 534
SICI:
0099-2240(200002)66:2<529:ITOSGL>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLETE NUCLEOTIDE-SEQUENCE; HYDROGEN AUTOTROPHY; MERCURY RESISTANCE; CHESAPEAKE-BAY; STRAINS; LIVIDANS; CHROMOSOME; HARBOR; GENES; PBL1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Hill, RT Univ Maryland, Inst Biotechnol, Ctr Marine Biotechnol, Columbus Ctr Suite 236,701 E Pratt St, Baltimore, MD 21202 USA Univ Maryland ColumbusCtr Suite 236,701 E Pratt St Baltimore MD USA 21202
Citazione:
J. Ravel et al., "Interspecific transfer of Streptomyces giant linear plasmids in sterile amended soil microcosms", APPL ENVIR, 66(2), 2000, pp. 529-534

Abstract

The interspecific transfer of two giant linear plasmids was investigated in sterile soil microcosms. Plasmids pRJ3L (322 kb) and pRJ28 (330 kb), bothencoding mercury resistance, were successfully transferred in amended soilmicrocosms from their streptomycete hosts, the isolates CHR3 and CHR28, respectively, to a plasmidless and mercury-sensitive strain, Streptomyces lividans TK24. Transconjugants of S. lividans TK24 were first observed after 2to 3 days of incubation at 30 degrees C, which corresponded to the time taken for the formation of mycelia in soil. Transfer frequencies were 4.8 x 10(-4) and 3.6 x 10(-5) CFU/donor genome for pRJ3L and pRJ28, respectively. Transconjugants were analyzed by pulsed-field gel electrophoresis for the presence of plasmids, and plasmid identity was confirmed by restriction digests. Total genomic DNA digests confirmed that transconjugants were S. lividans TK24. The mercury resistance genes were shown to be on the plasmid in the transconjugants by hybridization analysis and were still functional. This is the first demonstration of transfer of giant linear plasmids in sterile soil microcosms. Giant linear plasmids were detected in many Streptomycesspp. isolated from mercury-contaminated sediments from Boston Harbor (United States), Townsville Harbor (Australia), and the Sali River (Tucuman, Argentina). Mercury resistance genes were shown to be present on some of theseplasmids. Our findings that giant linear plasmids can be transferred between Streptomyces spp. and are common in environmental Streptomyces isolates suggest that these plasmids are important in gene transfer between streptomycetes in the environment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 22:17:59