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Titolo:
Production and characterisation of two monoclonal antibodies recognising equine IgG Fc receptors
Autore:
Aggarwal, N; Holmes, MA;
Indirizzi:
Univ Cambridge, Dept Clin Vet Med, Cambridge CB3 0ES, England Univ Cambridge Cambridge England CB3 0ES Med, Cambridge CB3 0ES, England
Titolo Testata:
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
fascicolo: 1, volume: 73, anno: 2000,
pagine: 63 - 71
SICI:
0165-2427(20000131)73:1<63:PACOTM>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
LARGE GRANULAR LYMPHOCYTES; NATURAL-KILLER CELLS; U937 CELLS; GAMMA-RII; ANTIGEN;
Keywords:
monoclonal antibodies; Fc gamma R; horse; IgG;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Aggarwal, N AFRC, Inst Anim Hlth, Pirbright Lab, Ash Rd, Woking GU24 0NF, Surrey, England AFRC Ash Rd Woking Surrey England GU24 0NF NF, Surrey, England
Citazione:
N. Aggarwal e M.A. Holmes, "Production and characterisation of two monoclonal antibodies recognising equine IgG Fc receptors", VET IMMUNOL, 73(1), 2000, pp. 63-71

Abstract

Despite the importance of IgG Fc receptors in the regulation of various immunological mechanisms, these receptors have not been well characterised inthe domesticated animals including equines. This paper describes the production of two monoclonal antibodies (CVS 59 and CVS 61) that recognise equine IgG Fc receptors. Fusions were conducted using BALB/c mice hyperimmunisedwith equine peripheral blood mononuclear cells. Hybridoma supernatants were screened on the basis of their ability to inhibit the resetting of equineantibody coated sheep erythrocytes with equine peripheral blood mononuclear cells. The mAbs were then characterised for cellular distribution of the antigen by flow cytometry. Using immunoprecipitation, it was shown that both under reducing and non-reducing conditions, CVS 59 and CVS 61 precipitated a 80 and 40 kD protein, respectively. From the functional study coupled with cellular distribution and molecular weight of the antigen recognised bythese mAbs, it would appear that CVS 59 and CVS 61 recognise an epitope onequine equivalent of human and murine Fc gamma RIII and Fc gamma RII, respectively. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:13:22