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Titolo:
MK-801 neurotoxicity in cupric silver-stained sections: Lesion reconstruction by 3-dimensional computer image analysis
Autore:
Fix, AS; Stitzel, SR; Ridder, GM; Switzer, RC;
Indirizzi:
Procter & Gamble Co, Miami Valley Labs, Cincinnati, OH 45253 USA Procter &Gamble Co Cincinnati OH USA 45253 abs, Cincinnati, OH 45253 USA
Titolo Testata:
TOXICOLOGIC PATHOLOGY
fascicolo: 1, volume: 28, anno: 2000,
pagine: 84 - 90
SICI:
0192-6233(200001/02)28:1<84:MNICSS>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CINGULATE RETROSPLENIAL CORTEX; DIZOCILPINE MALEATE; NEURONAL NECROSIS; RAT; DEGENERATION;
Keywords:
brain lesion; dizocilpine maleate; neuronal degeneration; neuropathology; neurotoxicant; N-methyl-D-aspartate (NMDA) antagonist;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Fix, AS Procter & Gamble Co, Miami Valley Labs, POB 538707, Cincinnati, OH45253 USA Procter & Gamble Co POB 538707 Cincinnati OH USA 45253 H 45253 USA
Citazione:
A.S. Fix et al., "MK-801 neurotoxicity in cupric silver-stained sections: Lesion reconstruction by 3-dimensional computer image analysis", TOX PATHOL, 28(1), 2000, pp. 84-90

Abstract

Routine histopathologic evaluation of the brain (paraffin embedding, hematoxylin and eosin staining) makes it difficult for an investigator to identify the overall location and relative extent of lesions as they relate to neural substructures. Moreover, it is very difficult to convey this information to others who are less familiar with neuroanatomy. This study combined a3-dimensional imaging program with a cupric silver stain for neuronal degeneration in order to determine the location and extent of a focal lesion produced by MK-801 (dizocilpine maleate), a glutamate receptor antagonist that induces necrosis in a small population of neurons in the cortex of rats. A male Sprague-Dawley rat was treated with a subcutaneous dose of MK-801 (10 mg/kg) and was perfused with fixative through the left ventricle 3 days after treatment, a time point known to reveal maximal neurotoxic effects. The brain was embedded in a gelatin matrix, frozen, and serially sectioned ata thickness of 40 mu m. The cupric silver method of de Olmos was used to stain frozen sections at 320-mu m intervals. Using a color charged-couple device (CCD) camera and a macro lens, a series of 3-dimensional images, whichencompassed the entire rostral to caudal extent of the brain, was captured. A computer program was written to define internal and external boundariesin these 2-dimensional images. Then, 3-dimensional reconstructions were generated on a Silicon Graphics workstation using IRIS "Explorer. " The quality of the 3-dimensional reconstructions allowed fur easy identification of various neural substructures while clearly revealing the exact location and extent of the resulting necrotic neurons that were positively identified bythe cupric silver stain. This 3-dimensional lesion reconstruction method provides a powerful tool fur conveying spatial information about the nature of neurotoxic lesions in the brain. In addition, it may be used to investigate further dose-response relationships and the effects of other neurotoxicants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:17:49