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Titolo:
Development of mouse dendritic cells from lineage-negative c-kit(low)pluripotent hemopoietic stem cells in vitro
Autore:
Feng, B; Inaba, M; Lian, ZX; Cui, YZ; Toki, J; Ito, T; Jin, TN; Fan, TX; Yang, GX; Yu, CZ; Kushida, T; Ikehara, S;
Indirizzi:
Kansai Med Univ, Dept Pathol 1, Moriguchi, Osaka 5708506, Japan Kansai MedUniv Moriguchi Osaka Japan 5708506 guchi, Osaka 5708506, Japan
Titolo Testata:
STEM CELLS
fascicolo: 1, volume: 18, anno: 2000,
pagine: 53 - 60
SICI:
1066-5099(2000)18:1<53:DOMDCF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; TUMOR-NECROSIS-FACTOR; HEMATOPOIETIC PROGENITOR CELLS; C-KIT-LIGAND; BONE-MARROW; LANGERHANS CELLS; FACTOR-ALPHA; IN-VITRO; LYMPHOID ORGANS; TNF-ALPHA;
Keywords:
c-kit(low); dendritic cells; hemopoietic stem cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Ikehara, S Kansai Med Univ, Dept Pathol 1, 10-15 Fumizono Cho, Moriguchi, Osaka 5708506, Japan Kansai Med Univ 10-15 Fumizono Cho Moriguchi Osaka Japan 5708506
Citazione:
B. Feng et al., "Development of mouse dendritic cells from lineage-negative c-kit(low)pluripotent hemopoietic stem cells in vitro", STEM CELLS, 18(1), 2000, pp. 53-60

Abstract

Dendritic cells (DCs) are essential for the presentation of antigens in the primary immune response. To examine the generation of DCs from hemopoietic stem cells in the bone marrow (BM), lineage-negative (Lin(-))/CD71(-) bone marrow cells (BMCs) from C57BL/6 mice were separated into major histocompatibility complex (MHC) class I-high/ c-kit(low) and MHC class I-high/c-kit(<low) (phenotypically c-kit-negative, but c-kit message only detected by reverse transcriptase-polymerase chain reaction) populations. A large numberof cells with the morphological, phenotypical, and functional characteristics of DCs was generated from both c-kit(low) and c-kit(low) populations when cultured with a combination of cytokines (GM-CSF, tumor necrosis factor-a [TNF-a], interleukin 7 [IL-7], IL-3, stem cell factor [SCF], and flt3 ligand); the cytokine combination studies revealed that SCF and IL-3 in addition to GM-CSF and TNF-a are essential for DCs to be generated from these primitive populations, To our surprise most (>80%) generated cells expressed high levels of DC surface markers such as DEC205 and MHC class II, and they were potent stimulators in the primary allogeneic T cell activation. The development of DCs from c-kit(<low) cells was slower than that from c-kit(low) cells. These results indicate that c-kit(low) cells are more primitive than c-kit(low) cells, although both c-kit(low) cells and c-kit(<low) cells can differentiate into DCs, It should be noted that the combination of thesecytokines selectively induces DCs from both c-kit(<low) and c-kit(low) cells in vitro, suggesting that the ex vivo expansion of DCs using these primitive cells would be applicable to immunotherapy.

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Documento generato il 26/09/20 alle ore 05:41:11