Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cryopreservation of in vitro-grown meristems of potato (Solanum tuberosum L.) by encapsulation-vitrification
Autore:
Hirai, D; Sakai, A;
Indirizzi:
Hokkaido Prefectural Plant Genet Resources Ctr, Takikawa, Hokkaido 0730013, Japan Hokkaido Prefectural Plant Genet Resources Ctr Takikawa Hokkaido Japan 0730013
Titolo Testata:
POTATO RESEARCH
fascicolo: 2, volume: 42, anno: 1999,
pagine: 153 - 160
SICI:
0014-3065(1999)42:2<153:COIVMO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHOOT-TIPS; APICAL MERISTEMS; SURVIVAL; GERMPLASM; FREEZE; CELLS;
Keywords:
osmoprotection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Hirai, D Hokkaido Prefectural Plant Genet Resources Ctr, 363-2 Minami Takinokawa, Takikawa, Hokkaido 0730013, Japan Hokkaido Prefectural Plant Genet Resources Ctr 363-2 Minami Takinokawa Takikawa Hokkaido Japan 0730013
Citazione:
D. Hirai e A. Sakai, "Cryopreservation of in vitro-grown meristems of potato (Solanum tuberosum L.) by encapsulation-vitrification", POTATO RES, 42(2), 1999, pp. 153-160

Abstract

Alginate coated meristems from in vitro-grown axillary buds of potato (Solanum tuberosum L.) were successfully cryopreserved by vitrification. Excised meristems were precultured on sucrose-enriched MS medium and then encapsulated. To induce dehydration tolerance (osmotolerance), encapsulated meristems were treated with a mixture of 2 M glycerol plus 0.6 M sucrose for 90 min. These encapsulated meristems were dehydrated with a highly concentratedvitrification solution (PVS2 solution) for 3 hr at 0 degrees C prior to a plunge into liquid nitrogen. Successfully vitrified meristems developed shoots within 3 weeks after plating without intermediary callus formation. Theaverage rate of shoot formation amounted to nearly 70%. No difference was observed in RAPD analysis using 17 primers between cryopreserved and non-treated plantlets. The cryogenic protocol was successfully applied to 14 cultivars. It was also confirmed that the encapsulated vitrified meristems produced much greater shoot formation than the encapsulated dried meristems. Thus, the encapsulation vitrification protocol appears promising for cryopreservation of potato germplasm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:53:12