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Titolo:
Male flower-specific expression of genes for polygalacturonase, pectin methylesterase and beta-1,3-glucanase in a dioecious willow (Salix gilgiana Seemen)
Autore:
Futamura, N; Mori, H; Kouchi, H; Shinohara, K;
Indirizzi:
Forestry & Forest Prod Res Inst, Bioresources Technol Div, Mol & Cell BiolSect, Ibaraki, Osaka 3058687, Japan Forestry & Forest Prod Res Inst Ibaraki Osaka Japan 3058687 058687, Japan Nagoya Univ, Grad Sch Bioagr Sci, Aichi 4648601, Japan Nagoya Univ AichiJapan 4648601 rad Sch Bioagr Sci, Aichi 4648601, Japan Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan Natl Inst Agrobiol Resources Tsukuba Ibaraki Japan 3058602 3058602, Japan
Titolo Testata:
PLANT AND CELL PHYSIOLOGY
fascicolo: 1, volume: 41, anno: 2000,
pagine: 16 - 26
SICI:
0032-0781(200001)41:1<16:MFEOGF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLLEN-SPECIFIC POLYGALACTURONASE; SILENE-LATIFOLIA; BRASSICA-NAPUS; SEQUENCE SIMILARITY; WHITE CAMPION; MESSENGER-RNA; TOBACCO GENE; MAIZE; PLANT; ENDOPOLYGALACTURONASE;
Keywords:
beta-1,3-glucanase; male flower; pectin methylesterase; pollen; polygalacturonase; willow (Salix gilgiana Seemen);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Shinohara, K Forestry & Forest Prod Res Inst, Bioresources Technol Div, Mol & Cell BiolSect, Ibaraki, Osaka 3058687, Japan Forestry & Forest Prod ResInst Ibaraki Osaka Japan 3058687
Citazione:
N. Futamura et al., "Male flower-specific expression of genes for polygalacturonase, pectin methylesterase and beta-1,3-glucanase in a dioecious willow (Salix gilgiana Seemen)", PLANT CEL P, 41(1), 2000, pp. 16-26

Abstract

We isolated cDNA clones (pSgPG1 through pSgPG4, pSgPME1 and pSgGN1) for the polygalacturonases (PGs), pectin methylesterase (PME) and beta-1,3-glucanase (GN) that are expressed specifically in male flowers of the dioecious willow (Salix gilgiana Seemen), The structural characteristics of the deduced proteins, designated SgPGs, SgPME1 and SgGN1, respectively, suggest that these enzymes function in pollens or anthers. The four SgPGs were more than91.9% homologous to one another at the amino acid level, indicating that their genes are members of a single family. Although the expression of the SgPGs, SgPME1 and SgGN1 was specific to male catkins (inflorescences), thesegenes were found in the genomes of both male and female plants. The expression of the transcripts of SgPGs, SgPME1 and SgGN1 was regulated developmentally in male reproductive organs. Maximal expression of SgPGs and SgPME1 was detected when male flowers were fully open and mature, while maximal expression of SgGN1 occurred at an earlier time. In situ hybridization revealed that the expression of SgPGs and SgPME1 was restricted to mature pollen grains after microspore mitosis. These results suggest that the pollen-specific or anther-specific expression of genes for PGs, PME and GN occurs in a dioecious plant, willow, just as it does in monoecious plants, and that theexpression of these genes is related to the developmental stage of pollen grains during male gametogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 12:19:16