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Titolo:
Anaerobic degradation and carbon isotopic fractionation of alkylbenzenes in crude oil by sulphate-reducing bacteria
Autore:
Wilkes, H; Boreham, C; Harms, G; Zengler, K; Rabus, R;
Indirizzi:
KFA Julich GmbH, Forschungszentrum, Inst Erdol & Organ Geochem, D-52425 Julich, Germany KFA Julich GmbH Julich Germany D-52425 Geochem, D-52425 Julich, Germany Australian Geol Survey Org, Canberra, ACT, Australia Australian Geol Survey Org Canberra ACT Australia nberra, ACT, Australia Max Planck Inst Marine Mikrobiol, D-28359 Bremen, Germany Max Planck Inst Marine Mikrobiol Bremen Germany D-28359 Bremen, Germany
Titolo Testata:
ORGANIC GEOCHEMISTRY
fascicolo: 1, volume: 31, anno: 2000,
pagine: 101 - 115
SICI:
0146-6380(2000)31:1<101:ADACIF>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYCLIC AROMATIC-HYDROCARBONS; AZOARCUS-TOLULYTICUS TOL-4; CHAIN ORGANIC-ACIDS; NORTH-SEA; ANOXIC CONDITIONS; FIELD WATERS; GROUNDWATER ENVIRONMENT; TOLUENE MINERALIZATION; DENITRIFYING BACTERIA; PETROLEUM RESERVOIRS;
Keywords:
sulphate-reducing bacteria; anaerobic biodegradation; alkylbenzenes; crude oils; carbon isotopic fractionation; benzoic acids; metabolite formation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Wilkes, H KFA Julich GmbH, Forschungszentrum, Inst Erdol & Organ Geochem, Postfach 1913, D-52425 Julich, Germany KFA Julich GmbH Postfach 1913 Julich Germany D-52425 h, Germany
Citazione:
H. Wilkes et al., "Anaerobic degradation and carbon isotopic fractionation of alkylbenzenes in crude oil by sulphate-reducing bacteria", ORG GEOCHEM, 31(1), 2000, pp. 101-115

Abstract

A mesophilic enrichment culture of sulphate-reducing bacteria isolated from the water phase of a North Sea oil tank using oil from the same tank as sole source of carbon and energy specifically depletes certain CL-CS alkylbenzenes in crude oil during growth. The enrichment culture grows on oils of different origin and composition resulting in similar patterns of alkylbenzene depletion. Two pure cultures of sulphate-reducing bacteria, strain oXyS1 and mXyS1 which were isolated on o-xylene and m-xylene, respectively, also grow on crude oil. Strain oXyS1 preferentially depletes o-xylene and o-ethyltoluene while strain mXyS1 preferentially depletes In-xylene and m-ethyltoluene. Roth strains also utilize toluene. The degradative patterns of thepure cultures are complementary and their combination results in the degradative pattern of the enrichment culture. During growth of the enrichment culture and the pure strains on crude oil alkylated benzoic acids were the main metabolic products, which were isolated from the water phases of the incubation experiments. The patterns of alkylated benzoic acids produced by the pure cultures are again complementary with respect to the pattern observed for the enrichment culture. The spectrum of alkylated benzoic acids suggests that partial oxidation of alkylbenzenes, which do not support growth, takes place resulting in the formation of dead-end metabolites. Alkylphenylsuccinic and fumaric acids were produced in trace amounts only. The portionof alkylbenzenes remaining in crude oil becomes enriched in C-13 during growth of bacteria. From the data obtained in this study it can be estimated that the carbon isotopic fractionation of the initial reaction of alkylbenzene degradation by the present bacteria is between -26 and -33 parts per thousand. We suggest that the variability in alkylbenzene concentrations and their carbon isotopic signature together with the occurrence of alkylated benzoic acids may be used as a specific indicator of initial biodegradation of crude oils and fossil fuel products by sulphate-reducing bacteria in various environments. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:14:31