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Titolo:
A novel Snail-related transcription factor Smuc regulates basic helix-loop-helix transcription factor activities via specific E-box motifs
Autore:
Kataoka, H; Murayama, T; Yokode, M; Mori, S; Sano, H; Ozaki, H; Yokota, Y; Nishikawa, S; Kita, T;
Indirizzi:
Kyoto Univ, Grad Sch Med, Dept Geriatr Med, Sakyo Ku, Kyoto 6068507, JapanKyoto Univ Kyoto Japan 6068507 riatr Med, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ, Grad Sch Med, Dept Mol Genet, Sakyo Ku, Kyoto 6068507, Japan Kyoto Univ Kyoto Japan 6068507 Mol Genet, Sakyo Ku, Kyoto 6068507, Japan
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 2, volume: 28, anno: 2000,
pagine: 626 - 633
SICI:
0305-1048(20000115)28:2<626:ANSTFS>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING PROPERTIES; ZINC-FINGER GENE; NEGATIVE REGULATOR; MESSENGER-RNAS; DROSOPHILA; PROTEIN; ENHANCER; REPRESSOR; ESCARGOT; MUSCLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Yokode, M Kyoto Univ, Grad Sch Med, Dept Geriatr Med, Sakyo Ku, 54 Kawahara Cho, Kyoto 6068507, Japan Kyoto Univ 54 Kawahara Cho Kyoto Japan 6068507o 6068507, Japan
Citazione:
H. Kataoka et al., "A novel Snail-related transcription factor Smuc regulates basic helix-loop-helix transcription factor activities via specific E-box motifs", NUCL ACID R, 28(2), 2000, pp. 626-633

Abstract

Snail family proteins are zinc finger transcriptional regulators first identified in Drosophila which play critical roles in cell fate determination. We identified a novel Snail-related gene from murine skeletal muscle cellsdesignated Smuc, Northern blot analysis showed that Smuc was highly expressed in skeletal muscle and thymus, Smuc contains five putative DNA-binding zinc finger domains in its C-terminal half. In electrophoretic mobility shift assays, recombinant zinc finger domains of Smuc specifically bound to CAGGTG and CACCTG E-box motifs (CANNTG). Because basic helix-loop-helix transcription factors (bHLH) bind to the same E-box sequences, we examined whether Smuc competes with the myogenic bHLH factor MyoD for DNA binding. Smuc inhibited the binding of a MyoD-E12 complex to the CACCTG E-box sequence in a dose-dependent manner and suppressed the transcriptional activity of MyoD-E12, When heterologously targeted to the thymidine kinase promoter as fusion proteins with the GAL4 DNA-binding domain, the non-zinc finger domain ofSmuc acted as a transcriptional repressor. Furthermore, overexpression of Smuc in myoblasts repressed transactivation of muscle differentiation marker Troponin T, Thus, Smuc might regulate bHLH transcription factors by zinc finger domains competing for E-box binding, and non-zinc finger repressor domains might also confer transcriptional repression to control differentiation processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 03:41:55