Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of [H-3]mazindol binding sites in cultured monkey amniotic epithelial cells
Autore:
Elwan, MA; Sakuragawa, N;
Indirizzi:
NCNP, Natl Inst Neurosci, Dept Inherited Metab Dis, Kodaira, Tokyo 1878502, Japan NCNP Kodaira Tokyo Japan 1878502 Metab Dis, Kodaira, Tokyo 1878502, Japan
Titolo Testata:
NEUROSCIENCE LETTERS
fascicolo: 1, volume: 279, anno: 2000,
pagine: 37 - 40
SICI:
0304-3940(20000121)279:1<37:CO[BSI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN DOPAMINE TRANSPORTER; MAZINDOL BINDING; H-3 MAZINDOL; NOREPINEPHRINE TRANSPORTERS; CATECHOLAMINES; NORADRENALINE; SEROTONIN; STRIATUM; CARRIER; RELEASE;
Keywords:
amniotic epithelial cells; dopamine transporter; [H-3]mazindol binding;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Sakuragawa, N NCNP, Natl Inst Neurosci, Dept Inherited Metab Dis, 4-1-1 Ogawahigashi, Kodaira, Tokyo 1878502, Japan NCNP 4-1-1 Ogawahigashi Kodaira Tokyo Japan 1878502 2, Japan
Citazione:
M.A. Elwan e N. Sakuragawa, "Characterization of [H-3]mazindol binding sites in cultured monkey amniotic epithelial cells", NEUROSCI L, 279(1), 2000, pp. 37-40

Abstract

Our previous studies showed that monkey amniotic epithelia I cells (MAEC) synthesize and release catecholamines a nd possess D1 and D2 dopamine (DA) receptors (Elwan, M.A., Ishii, T., Ono, F. and Sakuragawa, N., Evidence forthe presence of dopamine D1 receptor mRNA and binding sites in monkey amniotic epithelial cells. Neurosci. Lett., 262 (1999) 9-12; Elwan, M.A., Ishii, T. and Sakuragawa, N., Detection of dopamine D2 receptor mRNA and bindingsites in monkey amniotic epithelial cells. J. Neurosci. Res., 56 (1999) 316-322; Elwan, M.A., Thangavel, R., One, F. and Sakuragawa, N., Synthesis and release of catecholamines by cultured monkey amniotic epithelial cells. J. Neurosci. Res., 53 (1998) 107-113). In the present study we tested the presence of DA transporter (DAT) in MAEC using radioligand binding experiments. Saturation studies showed that [H-3]mazindol binds to a high affinity site with K-D and B-max values of 7.85 +/- 1.25 nM and 123.22 +/- 18.34 fmol/mg protein, respectively. Competition studies indicated that selective DAT inhibitors are potent displacers of [H-3]mazindol binding, compared to inhibitors of other types of transporters. The rank order of potency of the competing drugs is consistent with the pharmacology of DAT. These results provide, for the first time, clear evidence that MAEC natively possess DAT binding sites and suggest that MAEC may provide a potential primate cell model to study DA release and uptake processes and to explore new drugs active atthis site. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 18:07:01