Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CD5 is a potential selecting ligand for B-cell surface immunoglobulin: A possible role in maintenance and selective expansion of normal and malignantB cells
Autore:
Pospisil, R; Silverman, GJ; Marti, GE; Aruffo, A; Bowen, MA; Mage, RG;
Indirizzi:
NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA NIAID Bethesda MD USA 20892 AID, Immunol Lab, NIH, Bethesda, MD 20892 USA
Titolo Testata:
LEUKEMIA & LYMPHOMA
fascicolo: 3-4, volume: 36, anno: 2000,
pagine: 353 -
SICI:
1042-8194(200001)36:3-4<353:CIAPSL>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC LYMPHOCYTIC-LEUKEMIA; VARIABLE REGION GENES; CYSTEINE-RICH DOMAIN; MOLECULAR CHARACTERIZATION; ANTIGENIC EXPRESSION; LYMPHOID ORGANS; T-CELL; RECEPTOR; REPERTOIRE; HEAVY;
Keywords:
B-CLL; B-cell superantigens; CD5 domains; surface immunoglobulin; positive selection V-H framework regions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Mage, RG NIAID, Immunol Lab, NIH, Bldg 10,11N311 MSC 1892,10 Ctr Dr, Bethesda, MD 20892 USA NIAID Bldg 10,11N311 MSC 1892,10 Ctr Dr Bethesda MD USA 20892 USA
Citazione:
R. Pospisil et al., "CD5 is a potential selecting ligand for B-cell surface immunoglobulin: A possible role in maintenance and selective expansion of normal and malignantB cells", LEUK LYMPH, 36(3-4), 2000, pp. 353

Abstract

Although the function of CD5 on B cells is unknown, previous studies suggested that CD5 interaction with V-H framework regions of surface immunoglobulins (Igs) may contribute to survival and expansion of B cells, Here we used B-chronic lymphocytic leukemia (B-CLL) cells and transformed B-cell Linesfrom normal and B-CLL patients to study CD5-Ig interactions. Immobilized Ig binds and permits isolation of CD5 from lysates of CD5-expressing cell lines. Immunoglobulins or Fab fragments of different V-H families varied in their effective ness as inhibitors of anti-CD5 staining of CLL cells, appendix and tonsil tissue sections. Human Ig also binds to purified recombinant CD5. We show here for the first time that the unconventional Ig-CD5 interaction maps to the extracellular CD5-D2 domain whereas conventional epitopes recognized by anti-CD5 antibodies are localized in the D1 domain of CD5. Wepropose that interactions of V-H framework regions with CD5 as a ligand may maintain, select or expand normal, autoimmune or transformed B cells and also contribute to skewing of the normal V-H repertoire.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 01:29:43