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Titolo:
STOCHASTIC NATURE AND RED-CELL POPULATION-DISTRIBUTION OF THE SICKLING-INDUCED CA2+ PERMEABILITY
Autore:
LEW VL; ORTIZ OE; BOOKCHIN RM;
Indirizzi:
YESHIVA UNIV ALBERT EINSTEIN COLL MED,1300 MORRIS PK AVE BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED BRONX NY 10461 UNIV CAMBRIDGE,PHYSIOL LAB CAMBRIDGE CB2 EG ENGLAND
Titolo Testata:
The Journal of clinical investigation
fascicolo: 11, volume: 99, anno: 1997,
pagine: 2727 - 2735
SICI:
0021-9738(1997)99:11<2727:SNARPO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEOXYGENATION; ANEMIA; TRANSPORT; CALCIUM; HEMOGLOBIN; CHLORIDE; BAND-3; PUMP;
Keywords:
CA2+-ACTIVATED K-CHANNELS; SICKLE CELL ANEMIA; RED CELLS; VOLUME CONTROL; CA2+ PERMEABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
V.L. Lew et al., "STOCHASTIC NATURE AND RED-CELL POPULATION-DISTRIBUTION OF THE SICKLING-INDUCED CA2+ PERMEABILITY", The Journal of clinical investigation, 99(11), 1997, pp. 2727-2735

Abstract

To explore basic properties of the sickling-induced cation permeability pathway, the Ca2+ component (Psickle-Ca) was studied in density-fractionated sickle cell anemia (SS) discocytes through its effects on the activity of the cells' Ca2+-sensitive K+-channels (K-Ca). The instant state of K-Ca channel activation was monitored during continuous or cyclic deoxygenation of the cells using a novel thiocyanate-dense-cellformation method, Each deoxy pulse caused a reversible, sustained Psickle-Ca which activated K-Ca channels in only 10-45% of cells at physiological [Ca2+](0) (''activated cells''), After removal of cells activated by each previous deoxy pulse, subsequent pulses generated similaractivated cell fractions, indicating a random determination rather than the response of a specific vulnerable subpopulation, The fraction of activated cells rose monotonically with [Ca2+](0) along a curve reflecting the cells' distribution of Psickle-Ca, with values high enough in a small cell fraction to trigger near-maximal K-Ca channels, Consistent with the stochastic nature of Psickle-Ca, repeated deoxygenated-oxygenated pulsing led to progressive dense cell formation, whereas single long pulses caused one early density shift, Thus deoxygenation-induced Ca2+-permeabilization in SS cells is a probabilistic event with large cumulative dehydrating potential, The possible molecular nature of Psickle-Ca is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 08:15:05