Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Involvement of matrix metalloproteinases 2 and 9, tissue inhibitor of metalloproteinases and apoptosis in tissue remodelling in the sheep placenta
Autore:
Riley, SC; Webb, CJ; Leask, R; McCaig, FM; Howe, DC;
Indirizzi:
Univ Edinburgh, Ctr Reprod Biol, Dept Obstet & Gynaecol, Edinburgh EH3 9EW, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH3 9EW Midlothian, Scotland
Titolo Testata:
JOURNAL OF REPRODUCTION AND FERTILITY
fascicolo: 1, volume: 118, anno: 2000,
pagine: 19 - 27
SICI:
0022-4251(200001)118:1<19:IOMM2A>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HORMONE PRODUCTION; FETAL MEMBRANES; EARLY-PREGNANCY; GELATINASE-A; COLLAGENOLYSIS; INVASION; EWES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Riley, SC Univ Edinburgh, Ctr Reprod Biol, Dept Obstet & Gynaecol, Edinburgh EH3 9EW, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH3 9EW , Scotland
Citazione:
S.C. Riley et al., "Involvement of matrix metalloproteinases 2 and 9, tissue inhibitor of metalloproteinases and apoptosis in tissue remodelling in the sheep placenta", J REPR FERT, 118(1), 2000, pp. 19-27

Abstract

Placental growth and development is crucial for successful pregnancy. The aim of this study was to characterize the activity and localization of the matrix metalloproteinase 2 (MMP-2) and MMP-9, which are capable of degrading basement membrane collagen (predominantly collagen type IV), and their endogenous tissue inhibitor of matrix metalloproteinases (TIMPs), in amnioticfluid and in the developing ovine placenta. Cell deletion by apoptosis during placental development was also examined. Zymography with gelatin as substrate indicated that MMP-2 (72 kDa gelatinase A; predominantly latent form) was present in increasing amounts in amniotic fluid from day 70 of gestation to labour (days 140-145), and MMP-9 (92 kDa gelatinase B; predominantlylatent form) was detectable from day 125 to labour; there was no increase in MMP-2 or -9 in labour. A broad range of TIMPs was detected in amniotic fluid; the molecular masses corresponded to TIMP-1, -2 and -3. Immunohistochemical techniques localized MMP-2, MMP-9 and TIMP-3 in the sheep placenta, predominantly in the trophoblast layer in uninucleate, but not binucleate, cells. However, MMP-2 and -9 activated proteins in placental homogenates were low throughout pregnancy. Apoptosis was identified by morphological criteria and also by TdT-mediated dUTP nick end labelling. Apoptosis was present in discrete regions in the placenta, predominantly in trophoblast cells near the tips and the basal regions of the fetomaternal interdigitations. During pregnancy the sheep placenta becomes more complex and the area of the fetomaternal interface increases. MMP-2 and -9 are likely to be involved inbreaking down basement membranes to allow cell migration during this process. It is suggested that digestion of supporting extracellular matrix may trigger apoptosis and in some way increase the branching pattern in the villi.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:10:00