Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Does the sensory nucleus of the genitofemoral nerve have a role in testicular descent?
Autore:
Hrabovszky, Z; Farmer, PJ; Hutson, JM;
Indirizzi:
Royal Childrens Hosp, Inst Res, Melbourne, Vic, Australia Royal Childrens Hosp Melbourne Vic Australia , Melbourne, Vic, Australia
Titolo Testata:
JOURNAL OF PEDIATRIC SURGERY
fascicolo: 1, volume: 35, anno: 2000,
pagine: 96 - 100
SICI:
0022-3468(200001)35:1<96:DTSNOT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-RELATED-PEPTIDE; LUMBAR SPINAL-CORD; CREMASTER NUCLEUS; ANDROGEN BLOCKADE; RAT; FLUTAMIDE; GUBERNACULUM; CRYPTORCHIDISM; INNERVATION; CONTRACTILITY;
Keywords:
calcitonin gene-related peptide; genitofemoral nerve; dorsal root ganglia; testicular descent; sensory nerve;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Hutson, JM Royal Childrens Hosp, Flemington Rd, Parkville, Vic 3052, Australia Royal Childrens Hosp Flemington Rd Parkville Vic Australia 3052
Citazione:
Z. Hrabovszky et al., "Does the sensory nucleus of the genitofemoral nerve have a role in testicular descent?", J PED SURG, 35(1), 2000, pp. 96-100

Abstract

Background/Purpose: A role for the genitofemoral nerve (GFN) and its neurotransmitter, CORP, in testicular descent has been well established, The exact mechanism, however, by which circulating androgens act on the GFN is notyet known. The authors studied the sensory nucleus of the GFN (L1-L2 dorsal root ganglia [DRG]) to determine whether it is sexually dimorphic and able to be influenced by intrauterine antiandrogen treatment. Methods: Sprague-Dawley rats were injected daily with 100 mg/kg/d of the antiandrogen flutamide on day 16 to 19 of pregnancy. Control animals were treated with vehicle only. At the age of 2 to 3 days the newborn rats underwent unilateral dissection of the GFN. The proximal end was labelled with fluorescent dye, diamidinophenyl indole. The rats were killed 48 hours later, and the relevant ganglia (L1,L2) were removed. Cryostat frozen serial sections were cut, and retrogradely labelled fluorescent cells were counted under an epifluorescence microscope. In 32 animals, the cells were double fluorescent labelled with antibody to CORP and FITC. Results: Of 75 rats evaluated, the mean number of the DAPI-positive, retrogradely labelled cells in the control groups was 266 +/- 55 in the male, and 230 +/- 67 in the female as opposed to 186 +/- 45 and 161 +/- 35 in the flutamide-treated male and female groups, respectively. In 32 animals the DRG sections were double labelled for CORP. The number of CORP plus DAPI-positive cells were as follows: control males, 60 +/- 12; control females, 50 +/- 9; flutamide males, 36 +/- 8; flutamide females, 40 +/- 10. Conclusions: These findings show a sexual dimorphism in the number of GFN cell bodies in the DRG. Flutamide decreases the number of GFN cell bodies in the DRG of both males and females. Our results are consistent with a rolefor circulating androgens acting on the sensory nucleus of the GFN (DRG) instead of the motor nucleus as previously thought. The release of CORP fromthe nerve endings may occur via the sensory branch of the GFN.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:45:04