Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Relief of G-protein inhibition of calcium channels and short-term synapticfacilitation in cultured hippocampal neurons
Autore:
Brody, DL; Yue, DT;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Program Mol & Cellular Physiol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 hysiol, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 urosci, Baltimore, MD 21205 USA Johns Hopkins Univ, Sch Med, Progam Mol & Cellular Syst Physiol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 hysiol, Baltimore, MD 21205 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 3, volume: 20, anno: 2000,
pagine: 889 - 898
SICI:
0270-6474(20000201)20:3<889:ROGIOC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
SQUID GIANT SYNAPSE; EXCITATORY POSTSYNAPTIC CURRENTS; NEOCORTICAL PYRAMIDAL NEURONS; RAT SYMPATHETIC NEURONS; N-TYPE; PRESYNAPTIC INHIBITION; CA2+ CHANNELS; TRANSMITTER RELEASE; NERVE-TERMINALS; BETA-SUBUNIT;
Keywords:
short-term synaptic plasticity; facilitation; GABA; baclofen; G-protein inhibition; calcium channels; recombinant; calcium channels; microcultures; cultured neurons; autapses; hippocampal neurons; adenosine; HEK 293 cells;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
101
Recensione:
Indirizzi per estratti:
Indirizzo: Yue, DT Johns Hopkins Univ, Sch Med, Dept Biomed Engn, Program Mol & Cellular Physiol, 720 Rutland Ave, Baltimore, MD 21205 USA Johns Hopkins Univ 720 Rutland Ave Baltimore MD USA 21205 1205 USA
Citazione:
D.L. Brody e D.T. Yue, "Relief of G-protein inhibition of calcium channels and short-term synapticfacilitation in cultured hippocampal neurons", J NEUROSC, 20(3), 2000, pp. 889-898

Abstract

G-protein inhibition of voltage-gated calcium channels can be transiently relieved by repetitive physiological stimuli. Here, we provide evidence that such relief of inhibition contributes to short-term synaptic plasticity in microisland-cultured hippocampal neurons. With G-protein inhibition induced by the GABA(B) receptor agonist baclofen or the adenosine A1 receptor agonist 2-chloroadenosine, short-term synaptic facilitation emerged during action potential trains. The facilitation decayed with a time constant of similar to 100 msec. However, addition of the calcium channel inhibitor Cd2+ at 2-3 mu M had no such effect and did not alter baseline synaptic depression. As expected of facilitation from relief of channel inhibition, analysis of miniature EPSCs implicated presynaptic modulation, and elevating presynaptic Ca2+ entry blunted the facilitation. Most telling was the near occlusion of synaptic facilitation after selective blockade of P/Q- but not N-typecalcium channels. This was as predicted from experiments using recombinantcalcium channels expressed in human embryonic kidney (HEK) 293 cells; we found significantly stronger relief of G-protein inhibition in recombinant P/Q- versus N-type channels during action potential trains. G-protein inhibition in HEK 293 cells was induced via recombinant M2 muscarinic acetylcholine receptors activated by carbachol, an acetylcholine analog. Thus, relief of G-protein inhibition appears to produce a novel form of short-term synaptic facilitation in cultured neurons. Similar short-term synaptic plasticity may be present at a wide variety of synapses, as it could occur during autoreceptor inhibition by glutamate or GABA, heterosynaptic inhibition by GABA, tonic adenosine inhibition, and in many other instances.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 01:26:02