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Titolo:
The capacity to produce IFN-gamma rather than the presence of interleukin-4 determines the resistance and the degree of susceptibility to Leishmania donovani infection in mice
Autore:
Lehmann, J; Enssle, KH; Lehmann, I; Emmendorfer, A; Lohmann-Matthes, ML;
Indirizzi:
Fraunhofer Inst Toxicol & Aerosol Res, Dept Immunobiol, D-3000 Hannover, Germany Fraunhofer Inst Toxicol & Aerosol Res Hannover Germany D-3000 r, Germany Behringwerke AG, D-3550 Marburg, Germany Behringwerke AG Marburg GermanyD-3550 werke AG, D-3550 Marburg, Germany Univ Leipzig, Inst Clin Immunol & Transfus Med, D-7010 Leipzig, Germany Univ Leipzig Leipzig Germany D-7010 ransfus Med, D-7010 Leipzig, Germany
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 1, volume: 20, anno: 2000,
pagine: 63 - 77
SICI:
1079-9907(200001)20:1<63:TCTPIR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL VISCERAL LEISHMANIASIS; T-CELL SUBSETS; MURINE CUTANEOUS LEISHMANIASIS; INTERFERON-GAMMA; ACQUIRED-RESISTANCE; MONOCLONAL-ANTIBODY; IL-4 RECEPTOR; PROGRESSION; EXPRESSION; LYMPHOKINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Lehmann, J Univ Leipzig, Inst Immunol, Fac Vet Med, Tierklin 11, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 D-04103 Leipzig, Germany
Citazione:
J. Lehmann et al., "The capacity to produce IFN-gamma rather than the presence of interleukin-4 determines the resistance and the degree of susceptibility to Leishmania donovani infection in mice", J INTERF CY, 20(1), 2000, pp. 63-77

Abstract

The immune response against Leishmania donovani infection has been investigated in one resistant mouse strain (C3H/HeJ) and three susceptible mouse strains (C57BL/6, BALB/c, and B10D2/n), In order to correlate the strain-specific course of infection with the individual T cell response phenotype, the ex vivo cytokine secretion patterns of splenic lymphocytes were assessed by ELISA (interferon-gamma [IFN-gamma], interleukin-4 [IL-4], IL-10) or by bioassay (IL-2), The strain-dependent differences in the course of infection correlated closely with the potency of T cells to produce IFN-gamma, C3H/HeJ mice produced high amounts of IFN-gamma before and during infection, whereas susceptible mice produced low amounts of IFN-gamma early during L, donovani infection. However, C57BL/6 mice, which recovered from the infectionrapidly after the acute stage, developed marked IFN-gamma response within the first 30 days of infection. In contrast, in BALB/c and B10D2/n mice, the IFN-gamma production diminished during the acute stage, and this was associated with a delay in recovery and with subsequent switching into the chronic stage. Interestingly, CD8(+) T cells contributed significantly to IFN-gamma production during this phase. In contrast to IFN-gamma, the levels of IL-4 in response to antigen or mitogen ex vivo were always very low. Moreover, neutralization of endogenous IL-4 in vivo by treatment with soluble murine IL-4 receptor did not result in significant decreases in the parasite burdens in spleen and liver but did cause a decrease in the serum IgE level of L. donovani-infected BALB/c mice. These results confirm that in visceralleishmaniasis a Th1-dominated immune response is protective against the L.donovani parasites and, furthermore, that the capacity to produce IFN-gamma rather than the presence of IL-4 determines the efficacy of the immune response in susceptible mice. The data show that CD8(+) T cells represent an important source of IFN-gamma during L, donovani infection in susceptible mice, implying a role for this cell type in healing and development of protective immunity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:24:37