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Titolo:
Enantioselective analysis of metoprolol in plasma using high-performance liquid chromatographic direct and indirect separations: applications in pharmacokinetics
Autore:
Lanchote, VL; Bonato, PS; Cerqueira, PM; Pereira, VA; Cesarino, EJ;
Indirizzi:
USP, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, Brazil USP Ribeirao Preto Brazil BR-14040903 BC-14040903 Ribeirao Preto, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, Sao Paulo, Brazil Univ Sao Paulo Sao Paulo Brazil c Med Ribeirao Preto, Sao Paulo, Brazil
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 738, anno: 2000,
pagine: 27 - 37
SICI:
1387-2273(20000128)738:1<27:EAOMIP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHIRAL STATIONARY PHASE; BONDED ALPHA-1-ACID GLYCOPROTEIN; DIASTEREOMERIC DERIVATIVES; ENANTIOMERS; URINE; ASSAY; PHARMACODYNAMICS; PROPRANOLOL; METABOLISM; ANTAGONISTS;
Keywords:
enantiomer separation; metoprolol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Lanchote, VL USP, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Avenida Cafe S-N,Campus USP, BR-14040903 Ribeirao Preto, Brazil USP Avenida Cafe S-N,Campus USP Ribeirao Preto Brazil BR-14040903 BC
Citazione:
V.L. Lanchote et al., "Enantioselective analysis of metoprolol in plasma using high-performance liquid chromatographic direct and indirect separations: applications in pharmacokinetics", J CHROMAT B, 738(1), 2000, pp. 27-37

Abstract

Direct enantioselective separation on chiral stationary phases and indirect separation based on the formation of diastereomeric derivatives were developed and compared for the HPLC analysis of R(+) and S(-)-metoprolol in human plasma. Plasma samples prepared using solid-phase extraction columns or liquid-liquid extraction were directly analyzed on a Chiralpack(R) AD or ona Chiralcel(R) OD-H columns, respectively S-(-)-menthyl choroformate was also used to yield diastereomeric derivatives resolved on a RP-g column. Themethods were employed to determine plasma concentrations of metoprolol enantiomers in a pharmacokinetic study of single dose administration of racemic metoprolol to a healthy Caucasian volunteer phenotyped as extensive metabolizer of debrisoquine. The correlation coefficients among enantioselectivemetoprolol plasma concentrations (5-223 ng/ml) obtained by the three methods were equal or higher than 0.99. The direct method that employed the chiral column Chiralpak(R) AD may be considered the most sensitive, although the three methods demonstrated interchangeable use in the pharmacokinetic investigation. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:26:30