Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Measurement of intracellular (compartmental) pH by P-31 NMR in Aspergillusniger
Autore:
Hesse, SJA; Ruijter, GJG; Dijkema, C; Visser, J;
Indirizzi:
Agr Univ Wageningen, Dept Mol Phys, NL-6703 HA Wageningen, Netherlands AgrUniv Wageningen Wageningen Netherlands NL-6703 HA ingen, Netherlands Agr Univ Wageningen, Sect Mol Genet Ind Micoorganims, NL-6703 HA Wageningen, Netherlands Agr Univ Wageningen Wageningen Netherlands NL-6703 HA ingen, Netherlands
Titolo Testata:
JOURNAL OF BIOTECHNOLOGY
fascicolo: 1, volume: 77, anno: 2000,
pagine: 5 - 15
SICI:
0168-1656(20000128)77:1<5:MOI(PB>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; SACCHAROMYCES-CEREVISIAE; AIRLIFT BIOREACTOR; NEUROSPORA-CRASSA; CYTOPLASMIC PH; VACUOLAR PH; SPECTROSCOPY; YEAST; METABOLISM; ATPASE;
Keywords:
Aspergillus niger; intracellular pH; P-31 NMR; perfusion; immobilization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Hesse, SJA Agr Univ Wageningen, Dept Mol Phys, Dreijenlaan 3, NL-6703 HA Wageningen, Netherlands Agr Univ Wageningen Dreijenlaan 3 Wageningen Netherlands NL-6703 HA
Citazione:
S.J.A. Hesse et al., "Measurement of intracellular (compartmental) pH by P-31 NMR in Aspergillusniger", J BIOTECH, 77(1), 2000, pp. 5-15

Abstract

P-31 nuclear magnetic resonance (P-31 NMR) was used to monitor cytoplasmicand vacuolar pH values in the filamentous fungus Aspergillus niger. To obtain a homogeneous cell sample and to be able to perform long term in vivo NMR measurements A. niger mycelium was kept in a setup that allows perfusionof the cell plug within the NMR tube. Mycelial samples, however, became rapidly clogged during perfusion leading to (partial) anaerobiosis of the plug with subsequent acidification of the cytoplasm. As a result, only short-term NMR measurements (5-10 min) were possible using free mycelium. To increase and to prolong perfusion, A. niger was immobilized in Ca2+-alginate beads. Deteriorated spectra recorded under hypoxia could be completely restored in the presence of oxygen. With this system perfusion in the presence of citrate could be maintained for at least 18 h at much higher rates (15 ml min(-1) compared with 4 ml min(-1) for free mycelium). During this period P-31 NMR spectra were highly invariable, indicating approximate steady-state intracellular conditions during long term measurements. Perfusion in the presence of glucose resulted in complete depletion of the vacuolar inorganic phosphate pool within 45 min and yielded a higher pH gradient over the tonoplast than when citrate was used (Delta pH = 1.6 and 1.4, respectively). (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:17:56