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Titolo:
IMMUNOLOCALIZATION OF ESTROGEN RECEPTOR-ALPHA WITHIN THE TESTIS AND EXCURRENT DUCTS OF THE RAT AND MARMOSET MONKEY FROM PERINATAL LIFE TO ADULTHOOD
Autore:
FISHER JS; MILLAR MR; MAJDIC G; SAUNDERS PTK; FRASER HM; SHARPE RM;
Indirizzi:
MRC,REPROD BIOL UNIT,CTR REPROD BIOL,37 CHALMERS ST EDINBURGH EH3 9EWMIDLOTHIAN SCOTLAND MRC,REPROD BIOL UNIT,CTR REPROD BIOL EDINBURGH EH3 9EW MIDLOTHIAN SCOTLAND
Titolo Testata:
Journal of Endocrinology
fascicolo: 3, volume: 153, anno: 1997,
pagine: 485 - 495
SICI:
0022-0795(1997)153:3<485:IOERWT>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MALE REPRODUCTIVE-TRACT; ESTROGEN-RECEPTOR; ANDROGEN RECEPTORS; INTERSTITIAL-CELLS; EPITHELIAL-CELLS; EPIDIDYMIS; LEYDIG; MOUSE; LOCALIZATION; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
J.S. Fisher et al., "IMMUNOLOCALIZATION OF ESTROGEN RECEPTOR-ALPHA WITHIN THE TESTIS AND EXCURRENT DUCTS OF THE RAT AND MARMOSET MONKEY FROM PERINATAL LIFE TO ADULTHOOD", Journal of Endocrinology, 153(3), 1997, pp. 485-495

Abstract

The sites of action and the physiological role of oestrogens in the male reproductive tract are poorly understood. We have undertaken a systematic study of the immunoexpression of oestrogen receptor-alpha (ER alpha) in the male rat from late fetal life through to adulthood and compared the findings with results obtained in the marmoset monkey (Callithrix jacchus) from neonatal to adult life. The testes, rete testis,efferent ducts and epididymis were examined from normal male rats (aged 4, 8, 10, 15, 20, 25, 38, 48 and 90 days) and from male rat fetuseson days 17.5 and 18.5 of gestation; comparable tissues were examined from neonatal, infantile, peripubertal and adult marmosets aged 8, 18-24, 54-62 and 92-112 weeks respectively. Immunolocalisation of ER alpha used antigen retrieval and a monoclonal antibody directed to the N-terminus, which had proved superior to six other antisera tested. ER alpha was immunoexpressed in interstitial cells, including the fetal/neonatal generation of Leydig cells, in both the rat and marmoset. In therat, the adult generation of Leydig cells were also immunopositive for ER alpha whereas the comparable cells in the marmoset were only weakly immunopositive. ER alpha was not expressed in Sertoli cells, peritubular myoid cells, blood vessels or germ cells at any time in either species. In late fetal life in the rat, ER alpha was immunoexpressed incells surrounding the mesonephric tubules, whereas postnatally it wasexpressed in the epithelium of the rete testis and efferent ducts at all ages from 4 to 90 days; this immunoexpression was most pronounced in the efferent ducts. In the marmoset, the efferent ducts, but not the rete testis, also showed intense immunoexpression of ER alpha. Apartfrom sporadic immunostaining for ER alpha in the epididymal duct of the rat in the neonatal period, the caput, corpus and cauda epididymis were negative for immunoexpression of ER alpha at all ages in both species. These findings suggest that the main actions of oestrogens in the male reproductive tract, mediated by ER alpha, are related to the development and function of the efferent ducts and the Leydig cells. In consideration of data from this and previous studies of oestrogen binding, we predict possible sites of expression of other oestrogen receptors (e.g. ER beta) in Sertoli cells and the epididymis. Interactive effects, related to the relative levels of androgens and oestrogens, could be physiologically important in the excurrent ducts of the adult testis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:51:40